1. Identification of environmental staphylococci and coliforms by the Vitek Auto Microbic System
- Author
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M. Durrer, T. Kleiss, Y. Fuhrer, P. Deberghes, and J.-L. Cordier
- Subjects
Rapid identification ,Veterinary medicine ,fluids and secretions ,Micrococcaceae ,biology ,Identification device ,food and beverages ,bacteria ,Overall performance ,equipment and supplies ,biology.organism_classification ,Microbiology ,Food Science - Abstract
The Vitek Auto Microbic System (AMS) automated identification device was used in rapid identification of staphylococci and coliforms originating from food factory environments. From a total of 3324 isolates, an average of 76% were identified with a confidence > 50% (staphylococci: 61%, coliforms: 82%). AMS identifications of presumed staphylococci did not match well (24%) with ATB 32 STAPH identifications, but coliforms matched for 80% of the isolates compared with the API 20 E system. The differences observed between the AMS and ATB 32 STAPH in identifications could be due to inadequate isolation media, insufficient isolate purification, incorrect pre-screening of isolates or an incompetent AMS database. For coliforms, the overall performance proved sufficient for rapid screening of isolates from food factory bacterial ecology studies.
- Published
- 1995
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