1. Antioxidant properties and neuroprotective effects of isocampneoside II on hydrogen peroxide-induced oxidative injury in PC12 cells.
- Author
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Si CL, Shen T, Jiang YY, Wu L, Yu GJ, Ren XD, Xu GH, and Hu WC
- Subjects
- Animals, Apoptosis Regulatory Proteins biosynthesis, Apoptosis Regulatory Proteins genetics, Apoptosis Regulatory Proteins metabolism, Cell Survival drug effects, Chelating Agents pharmacology, Gene Expression Regulation drug effects, Hydrogen Peroxide antagonists & inhibitors, Hydrogen Peroxide toxicity, Nerve Tissue Proteins biosynthesis, Nerve Tissue Proteins genetics, Nerve Tissue Proteins metabolism, Neurons enzymology, Neurons metabolism, Oxidants antagonists & inhibitors, Oxidants toxicity, Oxidation-Reduction, Oxidoreductases metabolism, PC12 Cells, Rats, Reactive Oxygen Species antagonists & inhibitors, Reactive Oxygen Species metabolism, Antioxidants pharmacology, Apoptosis drug effects, Caffeic Acids pharmacology, Disaccharides pharmacology, Glycosides pharmacology, Lipid Peroxidation drug effects, Neurons drug effects, Neuroprotective Agents pharmacology, Oxidative Stress drug effects
- Abstract
Oxidative stress has been considered as a major cause of cell damage in various neurodegenerative disorders. One of the reasonable strategies for delaying the disease's progression is to prevent reactive oxygen species (ROS) mediated cellular injury by dietary or pharmaceutical augmentation of free radical scavengers. Isocampneoside II (ICD) is an active phenylethanoid glycoside isolated from the medicinal hardwood genus Paulownia. This study was designed to explore free radical scavenging potential of ICD in different in vitro systems and its protective role in hydrogen peroxide (H₂O₂)-induced oxidative stress and apoptotic death in cultured rat pheochromocytoma (PC12) cells. The results showed ICD eliminated approximately 80.75% superoxide radical at the concentration of 0.1mg/ml and inhibited metal chelating by 22.07% at 8 mg/ml. Additionally, ICD showed a strong ability on reducing power and provided protection against oxidative protein damage induced by hydroxyl radicals. Pretreatment of PC12 cells with ICD prior to H₂O₂ exposure elevated cell viability, enhanced activity of superoxide dismutase and catalase, and decreased levels of malondialdehyde and intracellular ROS. Furthermore, ICD inhibited cell apoptosis and Bax/Bcl-2 ratio induced by H₂O₂. These findings suggested ICD may be considered as a potential antioxidant agent and should encourage for further research in neurodegenerative diseases., (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Published
- 2013
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