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Antioxidant properties and neuroprotective effects of isocampneoside II on hydrogen peroxide-induced oxidative injury in PC12 cells.
- Source :
-
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2013 Sep; Vol. 59, pp. 145-52. Date of Electronic Publication: 2013 Jun 11. - Publication Year :
- 2013
-
Abstract
- Oxidative stress has been considered as a major cause of cell damage in various neurodegenerative disorders. One of the reasonable strategies for delaying the disease's progression is to prevent reactive oxygen species (ROS) mediated cellular injury by dietary or pharmaceutical augmentation of free radical scavengers. Isocampneoside II (ICD) is an active phenylethanoid glycoside isolated from the medicinal hardwood genus Paulownia. This study was designed to explore free radical scavenging potential of ICD in different in vitro systems and its protective role in hydrogen peroxide (H₂O₂)-induced oxidative stress and apoptotic death in cultured rat pheochromocytoma (PC12) cells. The results showed ICD eliminated approximately 80.75% superoxide radical at the concentration of 0.1mg/ml and inhibited metal chelating by 22.07% at 8 mg/ml. Additionally, ICD showed a strong ability on reducing power and provided protection against oxidative protein damage induced by hydroxyl radicals. Pretreatment of PC12 cells with ICD prior to H₂O₂ exposure elevated cell viability, enhanced activity of superoxide dismutase and catalase, and decreased levels of malondialdehyde and intracellular ROS. Furthermore, ICD inhibited cell apoptosis and Bax/Bcl-2 ratio induced by H₂O₂. These findings suggested ICD may be considered as a potential antioxidant agent and should encourage for further research in neurodegenerative diseases.<br /> (Copyright © 2013 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Apoptosis Regulatory Proteins biosynthesis
Apoptosis Regulatory Proteins genetics
Apoptosis Regulatory Proteins metabolism
Cell Survival drug effects
Chelating Agents pharmacology
Gene Expression Regulation drug effects
Hydrogen Peroxide antagonists & inhibitors
Hydrogen Peroxide toxicity
Nerve Tissue Proteins biosynthesis
Nerve Tissue Proteins genetics
Nerve Tissue Proteins metabolism
Neurons enzymology
Neurons metabolism
Oxidants antagonists & inhibitors
Oxidants toxicity
Oxidation-Reduction
Oxidoreductases metabolism
PC12 Cells
Rats
Reactive Oxygen Species antagonists & inhibitors
Reactive Oxygen Species metabolism
Antioxidants pharmacology
Apoptosis drug effects
Caffeic Acids pharmacology
Disaccharides pharmacology
Glycosides pharmacology
Lipid Peroxidation drug effects
Neurons drug effects
Neuroprotective Agents pharmacology
Oxidative Stress drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1873-6351
- Volume :
- 59
- Database :
- MEDLINE
- Journal :
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
- Publication Type :
- Academic Journal
- Accession number :
- 23770344
- Full Text :
- https://doi.org/10.1016/j.fct.2013.05.051