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Start Over Author "Biosynthèse Journal food and chemical toxicology : an international journal published for the british industrial biological research association
3 results on '"Biosynthèse '

1. Reduced toxicity of 3-epi-deoxynivalenol and de-epoxy-deoxynivalenol through deoxynivalenol bacterial biotransformation

Author

Wulf-Dieter Moll, Ana Paula Frederico Rodrigues Loureiro Bracarense, Juliana Rubira Gerez, Gerd Schatzmayr, Philippe Pinton, Isabelle P. Oswald, Ting Zhou, Alix Pierron, Laboratory of Animal Pathology, State University of Londrina = Universidade Estadual de Londrina, Biosynthèse & Toxicité des Mycotoxines (ToxAlim-BioToMyc), ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), BIOMIN Research Center, Agriculture and Agri-Food [Ottawa] (AAFC), This work was partially financed by the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Brazil and Comite Frances de Avaliacao da Cooperacao Universitaria com o Brasil (COFECUB), France, CAPES/COFECUB Program (number 0389/2019) and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil (number 308136/2018-7)., Biomin Research Center, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil, Comitê Francês de Avaliação da Cooperação Universitária com o Brasil (COFECUB), CAPES/COFECUB Program (number 0389/2019), and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil (number 308136/2018-7)

Subjects
Male, medicine.medical_specialty, Lymphoid Tissue, Swine, [SDV]Life Sciences [q-bio], [SDV.TOX.TVM]Life Sciences [q-bio]/Toxicology/Vegetal toxicology and mycotoxicology, Histopathology, Immunoglobulins, Food Contamination, [SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain, Biology, Toxicology, Occludin, Weight Gain, Microbiology, Immunomodulation, 03 medical and health sciences, chemistry.chemical_compound, 0404 agricultural biotechnology, Biotransformation, Detoxification, Intestine, Small, medicine, Ingestion, Animals, Mycotoxin, Adverse effect, 030304 developmental biology, 2. Zero hunger, 0303 health sciences, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, food and beverages, 04 agricultural and veterinary sciences, General Medicine, Organ Size, Mycotoxins, 040401 food science, Animal Feed, 3. Good health, chemistry, Liver, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Toxicity, Cytokines, Trichothecenes, Food Science
Abstract

International audience; Ingestion of deoxynivalenol (DON), one of the most common mycotoxin contaminants of cereals, leads to adverse effects for animal and human health. Bacterial biotransformation is a strategy to mitigate the toxicity of this mycotoxin. The present study aims to evaluate the toxicity of two bacterial biotranformation products of DON: 3-epi-deoxynivalenol (3-epi-DON) and de-epoxy-deoxynivalenol (DOM-1) through zootechnical, hematological, histological and immunological assays. Twenty-four 4weeks-old piglets received a control diet or a diet contaminated with 3 mg kg-1 DON, DOM-1, or 3-epi-DON for 7 days. Sample tissues were collected for histomorphometrical analysis, expression of cytokines and cell protein junctions. The zootechnical and hematological parameters were not modulated by any treatment. Ingestion of DON induced histological alterations in the intestine, liver and lymphoid organs, as well as an overexpression of pro-inflammatory cytokines, E-cadherin and occludin. These changes were not observed in piglets receiving the DOM-1 and 3-epi-DON contaminated diets. Pigs fed 3-epi-DON contaminated diet showed an increase in IgM levels in comparison with other diets, while no change was observed in IgA and IgG levels among the diets. Our results indicate that DOM-1 and 3-epi-DON are not toxic for piglets; thus bacterial biotransformation seems to be a sustainable alternative to reduce mycotoxin toxicity.

Published
2019

2. Combined hazard assessment of mycotoxins and their modified forms applying relative potency factors

Author

Marco Binaglia, Chiara Dall'Asta, Arno C. Gutleb, Manfred Metzler, Dominique Parent-Massin, Hans Steinkellner, Jan Alexander, Isabelle P. Oswald, European Food Safety Authority (EFSA), Department of Food and Drug, University of Parma = Università degli studi di Parma [Parme, Italie], Environmental Research and Innovation (ERIN) Department, Luxembourg Institute of Science and Technology (LIST), Institute of Applied Biosciences, Karlsruhe Institute of Technology (KIT), Biosynthèse & Toxicité des Mycotoxines (ToxAlim-BioToMyc), ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Université de Bretagne Occidentale, Norwegian institute for public health, Université de Bretagne Occidentale - UFR Sciences et Techniques (UBO UFR ST), Université de Brest (UBO), and Norwegian Institute of Public Health [Oslo] (NIPH)

Subjects
Tolerable daily intake, [SDV.TOX.TVM]Life Sciences [q-bio]/Toxicology/Vegetal toxicology and mycotoxicology, [SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain, Pharmacology, Toxicology, medicine.disease_cause, Risk Assessment, 03 medical and health sciences, chemistry.chemical_compound, Ttoxicity assessment, relative potency factors (RPFs), 0404 agricultural biotechnology, In vivo, medicine, T2-toxin (HT2), Potency, toxicity assessment, Animals, Humans, Mycotoxin, Mode of action, Zearalenone, 030304 developmental biology, 0303 health sciences, Reference dose, No-Observed-Adverse-Effect Level, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Chemistry, Toxin, fungi, modified forms, zearalenone (ZEN), Estrogens, 04 agricultural and veterinary sciences, General Medicine, 040401 food science, 3. Good health, T-2 Toxin, [SDV.TOX]Life Sciences [q-bio]/Toxicology, Food Science
Abstract

International audience; This paper describes a methodology for hazard assessment of groups of related substances for which toxicity data are insufficient, and which utilises, next to conventional toxicological assessments and mechanistic information, the derivation of relative toxicity potency factors (RPFs). Zearalenone (ZEN) and T-2 toxin (T2) and HT-2 toxin (HT2) and their modified forms have been used as examples. A tolerable daily intake (TDI) for ZEN of 0.25 µg/kg bw was established. In vitro and in vivo studies suggested that modified forms of ZEN act via the same mode of action as ZEN (oestrogenicity). Results from in vivo uterotrophic assays were used to establish RPFs, allowing inclusion the different modified forms in a group TDI with ZEN. A TDI for the sum of T2/HT2 of 0.02 µg/kg bw per day and an acute reference dose (ARfD) of 0.3 µg/kg bw for the sum of T2/HT2 was established. In vitro studies show that phase I metabolites of T2/HT2 act via a similar mode of action as their parent compounds, namely protein synthesis inhibition with immune-and haematotoxicity. The phase I *Manuscript for revision (track changes hidden) Click here to view linked References 2 metabolites as well as conjugates of T2/HT2 and their phase I metabolites can be included in a group TDI with T2/HT2 applying RPFs.

Published
2019

3. Characterization of the contribution of buccal absorption to internal exposure to bisphenol A through the diet

Author

Véronique Gayrard, Sylvie Puel, Marlène Z. Lacroix, Nicole Picard-Hagen, Glenn Gauderat, Davy Guignard, Pierre-Louis Toutain, Catherine Viguié, ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Exposition, Perturbation Endocrino-métabolique et Reproduction (ToxAlim-EXPER), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3), and Biosynthèse & Toxicité des Mycotoxines (ToxAlim-BioToMyc)

Subjects
0301 basic medicine, Bisphenol A, endocrine system, Metabolite, bisphenol A, [SDV]Life Sciences [q-bio], Physiology, Oral Mucosal Absorption, Absorption (skin), 010501 environmental sciences, Toxicology, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, Dogs, Pharmacokinetics, Phenols, Animals, food toxicology, Tissue Distribution, Buccal Absorption, pharmacokinetic, Benzhydryl Compounds, 0105 earth and related environmental sciences, Sheep, urogenital system, Mouth Mucosa, General Medicine, Buccal administration, Free Radical Scavengers, Bioavailability, Diet, 030104 developmental biology, chemistry, hormones, hormone substitutes, and hormone antagonists, Food Science, buccal absorption
Abstract

International audience; The gavage route is often used for the toxicological evaluation of food contaminants. This route does not take into account absorption of the toxicants through the buccal mucosa, as evidenced in dogs for bisphenol A (BPA). Our goal was to determine the functional significance of buccal BPA absorption during dietary exposure. Four ewes received BPA by nasogastric gavage (100 mg/kg) and through food pellets (10 mg/kg), 13 days apart. The time course of serum concentrations of BPA and its main metabolite BPA-G was submitted to non-compartmental analysis. The dietary route led to 3-fold higher bioavailability as compared to gavage. The ratio of BPA-G to BPA concentrations varied greatly over time after the food administration, but not after gavage, suggesting a delayed metabolism of BPA after dietary exposure. The maximum entrance rate of BPA in the systemic circulation, determined by deconvolution analysis, was much higher after dietary administration than after gavage and a biphasic pattern of BPA entry was observed in 3 of the 4 ewes. Our results evidenced a dual mechanism of BPA absorption (buccal and digestive) after dietary exposure and highlight the necessity to take buccal absorption into account when evaluating food contaminants.

Published
2016

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