1. The indole alkaloids from the roots of Isatidis Radix
- Author
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Xianbang Hou, Huiqin Xu, Jingting Bi, Jing Chen, Liwei He, Qiangzi Zhang, and Faqin Wang
- Subjects
Male ,China ,Phytochemicals ,Isatis indigotica ,Isatidis Radix ,01 natural sciences ,Antiviral Agents ,Plant Roots ,Indole Alkaloids ,Rats, Sprague-Dawley ,Liquid chromatography–mass spectrometry ,Drug Discovery ,Animals ,Isatis ,Pharmacology ,Indole test ,Chromatography ,Molecular Structure ,010405 organic chemistry ,Chemistry ,Antithrombin Activity ,General Medicine ,Radix isatidis ,In vitro ,0104 chemical sciences ,Rats ,Molecular Docking Simulation ,010404 medicinal & biomolecular chemistry ,Drugs, Chinese Herbal - Abstract
The root of Isatis indigotica is used as a traditional Chinese medicine (termed Isatidis Radix) due to its antiviral effects. We examined compounds isolated from Isatidis Radix and elucidated the structures of three new natural alkaloids, and we examined the possible mechanisms or active targets of indole alkaloids occurring in blood of rats treated by gavage. Three new natural products were isolated from Radix Isatidis for the first time, including 1-methoxy-2-indoleacetonitrile, 1-hydroxy-3-indoleacetonitrile, 8-Methoxy-1, 2-dihydroquinoline, and 4 compounds isolated from this medicinal material for the first time. Their structures were elucidated using nuclear magnetic resonance. The components of Isatidis Radix were analysed using liquid chromatography tandem mass spectrometry, and 33 compounds were detected in plasma of treated rats; 24 of these compounds were indole alkaloids, and they included the newly identified compounds. Molecular docking and in vitro antithrombin activity tests showed HA inhibition activity of indoleacetonitriles.
- Published
- 2021