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The indole alkaloids from the roots of Isatidis Radix
- Source :
- Fitoterapia. 153
- Publication Year :
- 2021
-
Abstract
- The root of Isatis indigotica is used as a traditional Chinese medicine (termed Isatidis Radix) due to its antiviral effects. We examined compounds isolated from Isatidis Radix and elucidated the structures of three new natural alkaloids, and we examined the possible mechanisms or active targets of indole alkaloids occurring in blood of rats treated by gavage. Three new natural products were isolated from Radix Isatidis for the first time, including 1-methoxy-2-indoleacetonitrile, 1-hydroxy-3-indoleacetonitrile, 8-Methoxy-1, 2-dihydroquinoline, and 4 compounds isolated from this medicinal material for the first time. Their structures were elucidated using nuclear magnetic resonance. The components of Isatidis Radix were analysed using liquid chromatography tandem mass spectrometry, and 33 compounds were detected in plasma of treated rats; 24 of these compounds were indole alkaloids, and they included the newly identified compounds. Molecular docking and in vitro antithrombin activity tests showed HA inhibition activity of indoleacetonitriles.
- Subjects :
- Male
China
Phytochemicals
Isatis indigotica
Isatidis Radix
01 natural sciences
Antiviral Agents
Plant Roots
Indole Alkaloids
Rats, Sprague-Dawley
Liquid chromatography–mass spectrometry
Drug Discovery
Animals
Isatis
Pharmacology
Indole test
Chromatography
Molecular Structure
010405 organic chemistry
Chemistry
Antithrombin Activity
General Medicine
Radix isatidis
In vitro
0104 chemical sciences
Rats
Molecular Docking Simulation
010404 medicinal & biomolecular chemistry
Drugs, Chinese Herbal
Subjects
Details
- ISSN :
- 18736971
- Volume :
- 153
- Database :
- OpenAIRE
- Journal :
- Fitoterapia
- Accession number :
- edsair.doi.dedup.....ccbfb29305d7251a57a826339023017a