1. The protective effects of vernicilignan A, a new flavonolignan isolated from Toxicodendron vernicifluum on SH-SY5Y cells against oxidative stress-induced injury.
- Author
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Li, Meichen, Fan, Yanhua, Zhong, Ting, Yi, Ping, Fan, Chengcheng, Wang, Andong, Liu, Jianyu, and Xu, Yongnan
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PROTEIN metabolism , *APOPTOSIS , *CELL culture , *CELL lines , *CELLULAR signal transduction , *CHROMATOGRAPHIC analysis , *FLAVONOIDS , *HIGH performance liquid chromatography , *HYDROGEN peroxide , *LACTATE dehydrogenase , *MEDICINAL plants , *NEURODEGENERATION , *NUCLEAR magnetic resonance spectroscopy , *RESEARCH funding , *STAINS & staining (Microscopy) , *THIN layer chromatography , *WESTERN immunoblotting , *PLANT extracts , *OXIDATIVE stress , *CELL survival - Abstract
In this study, a new flavonolignan vernicilignan A was isolated from Toxicodendron vernicifluum. The neuroprotective effects of this compound against H 2 O 2 induced cell injury in SH-SY5Y cells were evaluated by MTT assay and LDH release assay. Vernicilignan A dose-dependently attenuated the cell injury and LDH release induced by H 2 O 2 in SH-SY5Y cells. Further study indicated that vernicilignan A reduced cell apoptosis caused by H 2 O 2 treatment via regulation of some apoptotic related proteins including Bax, Bcl-2, caspase 3 and caspase 9. Also, vernicilignan A increase the cell viability of H 2 O 2 treated cells via the activation of Akt and GSK3β. Base on the findings, vernicilignan A exhibited neuroprotective effects through the activation of PI3K/Akt signaling and inhibition of mitochondria apoptosis pathway. Vernicilignan A might be a promising therapeutic agent for oxidative stress induced neurodegenerative diseases. Unlabelled Image [ABSTRACT FROM AUTHOR]
- Published
- 2019
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