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The protective effects of vernicilignan A, a new flavonolignan isolated from Toxicodendron vernicifluum on SH-SY5Y cells against oxidative stress-induced injury.
- Source :
-
Fitoterapia . Apr2019, Vol. 134, p81-87. 7p. - Publication Year :
- 2019
-
Abstract
- In this study, a new flavonolignan vernicilignan A was isolated from Toxicodendron vernicifluum. The neuroprotective effects of this compound against H 2 O 2 induced cell injury in SH-SY5Y cells were evaluated by MTT assay and LDH release assay. Vernicilignan A dose-dependently attenuated the cell injury and LDH release induced by H 2 O 2 in SH-SY5Y cells. Further study indicated that vernicilignan A reduced cell apoptosis caused by H 2 O 2 treatment via regulation of some apoptotic related proteins including Bax, Bcl-2, caspase 3 and caspase 9. Also, vernicilignan A increase the cell viability of H 2 O 2 treated cells via the activation of Akt and GSK3β. Base on the findings, vernicilignan A exhibited neuroprotective effects through the activation of PI3K/Akt signaling and inhibition of mitochondria apoptosis pathway. Vernicilignan A might be a promising therapeutic agent for oxidative stress induced neurodegenerative diseases. Unlabelled Image [ABSTRACT FROM AUTHOR]
- Subjects :
- *PROTEIN metabolism
*APOPTOSIS
*CELL culture
*CELL lines
*CELLULAR signal transduction
*CHROMATOGRAPHIC analysis
*FLAVONOIDS
*HIGH performance liquid chromatography
*HYDROGEN peroxide
*LACTATE dehydrogenase
*MEDICINAL plants
*NEURODEGENERATION
*NUCLEAR magnetic resonance spectroscopy
*RESEARCH funding
*STAINS & staining (Microscopy)
*THIN layer chromatography
*WESTERN immunoblotting
*PLANT extracts
*OXIDATIVE stress
*CELL survival
Subjects
Details
- Language :
- English
- ISSN :
- 0367326X
- Volume :
- 134
- Database :
- Academic Search Index
- Journal :
- Fitoterapia
- Publication Type :
- Academic Journal
- Accession number :
- 136135322
- Full Text :
- https://doi.org/10.1016/j.fitote.2019.01.011