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4. Functional expression of the mutants of the chloroplast tRNA(Lys) gene from the liverwort, Marchantia polymorpha, in Escherichia coli.

Author

Nakahigashi K, Inokuchi H, and Ozeki H

Subjects
Base Composition, Base Sequence, Chloroplasts metabolism, Cloning, Molecular, Exons, Gene Expression, Introns, Molecular Sequence Data, Nucleic Acid Conformation, Oligonucleotide Probes, Suppression, Genetic, Escherichia coli genetics, Genes, Plant, Mutation, Plants genetics, RNA, Transfer, Amino Acid-Specific genetics, RNA, Transfer, Lys genetics
Abstract

The anticodon of the tRNA(Lys) gene (trnK) in the liverwort, Marchantia polymorpha, was artificially converted to an amber anticodon. This mutant tRNA(Lys) (CTA) gene carrying either the intron of the C27-C43 mismatch at the anticodon-stem is not functional in Escherichia coli, but without both of them, it does work as a tRNA(Lys) amber suppressor.

Published
1990
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