Functional expression of the mutants of the chloroplast tRNA(Lys) gene from the liverwort, Marchantia polymorpha, in Escherichia coli.

Authors :: Nakahigashi K
Inokuchi H
Ozeki H
Source :: FEBS letters [FEBS Lett] 1990 Jun 04; Vol. 265 (1-2), pp. 59-62.
Publication Year :: 1990
Abstract: The anticodon of the tRNA(Lys) gene (trnK) in the liverwort, Marchantia polymorpha, was artificially converted to an amber anticodon. This mutant tRNA(Lys) (CTA) gene carrying either the intron of the C27-C43 mismatch at the anticodon-stem is not functional in Escherichia coli, but without both of them, it does work as a tRNA(Lys) amber suppressor.

Subjects :: Base Composition
Base Sequence
Chloroplasts metabolism
Cloning, Molecular
Exons
Gene Expression
Introns
Molecular Sequence Data
Nucleic Acid Conformation
Oligonucleotide Probes
Suppression, Genetic
Escherichia coli genetics
Genes, Plant
Mutation
Plants genetics
RNA, Transfer, Amino Acid-Specific genetics
RNA, Transfer, Lys genetics

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