1. Mast cell-derived TNF-α and histamine modify IL-6 and IL-8 expression and release from cutaneous tumor cells.
- Author
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Artuc M, Guhl S, Babina M, Unger T, Steckelings UM, and Zuberbier T
- Subjects
- Antibodies immunology, Antibodies pharmacology, Carcinoma, Squamous Cell metabolism, Carcinoma, Squamous Cell pathology, Cell Line, Tumor, Cells, Cultured, Coculture Techniques methods, Culture Media, Conditioned pharmacology, Gene Expression drug effects, Gene Expression genetics, Histamine pharmacology, Histamine H1 Antagonists pharmacology, Histamine H2 Antagonists pharmacology, Humans, Interleukin-6 genetics, Interleukin-8 genetics, Melanoma metabolism, Melanoma pathology, Skin Neoplasms pathology, Tumor Necrosis Factor-alpha immunology, Histamine metabolism, Interleukin-6 metabolism, Interleukin-8 metabolism, Mast Cells metabolism, Skin Neoplasms metabolism, Tumor Necrosis Factor-alpha metabolism
- Abstract
The coincidence of skin tumors and elevated mast cell (MC) numbers has been known for many years. However, it has remained controversial whether, in this context, MCs promote or inhibit tumor growth. Addressing this problem, different melanoma and squamous cell carcinoma cell lines were co-cultivated with primary, dermal MC for 24 h and gene or protein expression of cytokines tumor necrosis factor (TNF-α), interleukin-6 (IL-6) and interleukin-8 (IL-8) estimated. Co-culture with MCs led to an increase in IL-8 gene expression and IL-8 protein release from melanoma cells and IL-6 and IL-8 gene expression and protein release from squamous cell carcinoma cells, respectively. Moreover induction of IL-6 and IL-8 was primarily regulated by MC-derived TNF-α. Our data suggest an interplay between MCs and tumor cells, which results in altered cytokine release and may, thus, have an impact on tumor growth, invasion and neovascularisation., (© 2011 John Wiley & Sons A/S.)
- Published
- 2011
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