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Mast cell-derived TNF-α and histamine modify IL-6 and IL-8 expression and release from cutaneous tumor cells.
- Source :
-
Experimental dermatology [Exp Dermatol] 2011 Dec; Vol. 20 (12), pp. 1020-2. Date of Electronic Publication: 2011 Oct 13. - Publication Year :
- 2011
-
Abstract
- The coincidence of skin tumors and elevated mast cell (MC) numbers has been known for many years. However, it has remained controversial whether, in this context, MCs promote or inhibit tumor growth. Addressing this problem, different melanoma and squamous cell carcinoma cell lines were co-cultivated with primary, dermal MC for 24 h and gene or protein expression of cytokines tumor necrosis factor (TNF-α), interleukin-6 (IL-6) and interleukin-8 (IL-8) estimated. Co-culture with MCs led to an increase in IL-8 gene expression and IL-8 protein release from melanoma cells and IL-6 and IL-8 gene expression and protein release from squamous cell carcinoma cells, respectively. Moreover induction of IL-6 and IL-8 was primarily regulated by MC-derived TNF-α. Our data suggest an interplay between MCs and tumor cells, which results in altered cytokine release and may, thus, have an impact on tumor growth, invasion and neovascularisation.<br /> (© 2011 John Wiley & Sons A/S.)
- Subjects :
- Antibodies immunology
Antibodies pharmacology
Carcinoma, Squamous Cell metabolism
Carcinoma, Squamous Cell pathology
Cell Line, Tumor
Cells, Cultured
Coculture Techniques methods
Culture Media, Conditioned pharmacology
Gene Expression drug effects
Gene Expression genetics
Histamine pharmacology
Histamine H1 Antagonists pharmacology
Histamine H2 Antagonists pharmacology
Humans
Interleukin-6 genetics
Interleukin-8 genetics
Melanoma metabolism
Melanoma pathology
Skin Neoplasms pathology
Tumor Necrosis Factor-alpha immunology
Histamine metabolism
Interleukin-6 metabolism
Interleukin-8 metabolism
Mast Cells metabolism
Skin Neoplasms metabolism
Tumor Necrosis Factor-alpha metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1600-0625
- Volume :
- 20
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Experimental dermatology
- Publication Type :
- Report
- Accession number :
- 21995333
- Full Text :
- https://doi.org/10.1111/j.1600-0625.2011.01377.x