1. Complement C6 and C7 DNA polymorphisms analysed by PCR in seven ethnic groups and characterisation of the C6 MspI RFLP.
- Author
-
Fernie BA, Finlay A, Price D, Chan E, Orren A, Joysey VC, Joysey KA, and Hobart MJ
- Subjects
- Alleles, China ethnology, Europe ethnology, Gene Frequency immunology, Haplotypes immunology, Humans, India ethnology, Malaysia ethnology, Polymerase Chain Reaction, South Africa ethnology, Spain ethnology, Complement C6 genetics, Complement C7 genetics, DNA isolation & purification, Deoxyribonuclease HpaII genetics, Polymorphism, Genetic, Polymorphism, Restriction Fragment Length
- Abstract
Five polymorphisms in the C6 and C7 genes have been investigated in seven ethnic groups. The allele frequencies are broadly similar in most groups except C7 M/N which is monomorphic in our group of Africans, and C6 MspI and C7 S367T where the allele frequencies in African and Cape Coloured subjects are very different from the other ethnic groups. There is very little allelic association except between C6 A/B and C6 MspI. Seventeen of the 32 possible haplotypes have been observed, suggesting that much recombination has taken place. We describe a new method for the investigation of the MspI RFLP located in intron 3 of C6 (approximately 3 kbp 3' from exon 3 and 1.5 kbp 5' from exon 4) and its molecular basis, together with an improved method for the isolation of DNA from stored serum.
- Published
- 1996