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Complement C6 and C7 DNA polymorphisms analysed by PCR in seven ethnic groups and characterisation of the C6 MspI RFLP.
- Source :
-
Experimental and clinical immunogenetics [Exp Clin Immunogenet] 1996; Vol. 13 (2), pp. 92-103. - Publication Year :
- 1996
-
Abstract
- Five polymorphisms in the C6 and C7 genes have been investigated in seven ethnic groups. The allele frequencies are broadly similar in most groups except C7 M/N which is monomorphic in our group of Africans, and C6 MspI and C7 S367T where the allele frequencies in African and Cape Coloured subjects are very different from the other ethnic groups. There is very little allelic association except between C6 A/B and C6 MspI. Seventeen of the 32 possible haplotypes have been observed, suggesting that much recombination has taken place. We describe a new method for the investigation of the MspI RFLP located in intron 3 of C6 (approximately 3 kbp 3' from exon 3 and 1.5 kbp 5' from exon 4) and its molecular basis, together with an improved method for the isolation of DNA from stored serum.
- Subjects :
- Alleles
China ethnology
Europe ethnology
Gene Frequency immunology
Haplotypes immunology
Humans
India ethnology
Malaysia ethnology
Polymerase Chain Reaction
South Africa ethnology
Spain ethnology
Complement C6 genetics
Complement C7 genetics
DNA isolation & purification
Deoxyribonuclease HpaII genetics
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Subjects
Details
- Language :
- English
- ISSN :
- 0254-9670
- Volume :
- 13
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Experimental and clinical immunogenetics
- Publication Type :
- Academic Journal
- Accession number :
- 9063701