1. The zebrafish galectins Drgal1-L2 and Drgal3-L1 bind in vitro to the infectious hematopoietic necrosis virus (IHNV) glycoprotein and reduce viral adhesion to fish epithelial cells
- Author
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Chiguang Feng, Stuart M. Haslam, Mihai Nita-Lazar, Vikram N. Vakharia, Hafiz Ahmed, Anne Dell, Barbara Giomarelli, Shawn Jackson, Chinnarajan Ravindran, Gerardo R. Vasta, Nuria González-Montalbán, Justin Mancini, Arun Ammayappan, Ana de las Heras-Sánchez, Gang Wu, and Biotechnology and Biological Sciences Research Council (BBSRC)
- Subjects
0301 basic medicine ,DIFFERENTIAL RECOGNITION ,Viral Envelope Proteins ,ADAPTIVE IMMUNITY ,MOLECULAR CHARACTERIZATION ,Zebrafish ,Cells, Cultured ,chemistry.chemical_classification ,Infectivity ,Novirhabdovirus ,DANIO-RERIO ,Virulence ,biology ,Cell biology ,1107 Immunology ,Host-Pathogen Interactions ,Galectin ,ANGUILLA-JAPONICA ,PERKINSUS-MARINUS ,Life Sciences & Biomedicine ,Infectious hematopoietic necrosis virus ,animal structures ,Galectins ,Recombinant Fusion Proteins ,Immunology ,Viral adhesion ,Virus Attachment ,IHNV envelope glycoprotein ,Article ,03 medical and health sciences ,Chimera (genetics) ,Rhabdoviridae Infections ,Animals ,Infectious hematopoietic necrosis virus (IHNV) ,Science & Technology ,030102 biochemistry & molecular biology ,OYSTER CRASSOSTREA-VIRGINICA ,Epithelial Cells ,Zebrafish Proteins ,Rhabdoviridae ,SKIN MUCUS ,biology.organism_classification ,Virology ,030104 developmental biology ,chemistry ,MONOCLONAL-ANTIBODIES ,HOST-CELLS ,Glycoprotein ,Zoology ,Developmental Biology - Abstract
The infectious hematopoietic necrosis virus (IHNV; Rhabdoviridae, Novirhabdovirus) infects teleost fish, such as salmon and trout, and is responsible for significant losses in the aquaculture industry and in wild fish populations. Although IHNV enters the host through the skin at the base of the fins, the viral adhesion and entry mechanisms are not fully understood. In recent years, evidence has accumulated in support of the key roles played by protein-carbohydrate interactions between host lectins secreted to the extracellular space and virion envelope glycoproteins in modulating viral adhesion and infectivity. In this study, we assessed in vitro the potential role(s) of zebrafish (Danio rerio) proto type galectin-1 (Drgal1-L2) and a chimera galectin-3 (Drgal3-L1) in IHNV adhesion to epithelial cells. Our results suggest that the extracellular Drgal1-L2 and Drgal3-L1 interact directly and in a carbohydrate-dependent manner with the IHNV glycosylated envelope and glycans on the epithelial cell surface, significantly reducing viral adhesion.
- Published
- 2016