1. Further studies on short-term adaptations in the expression of lipogenic genes in broilers.
- Author
-
Rosebrough RW, Russell BA, and Richards MP
- Subjects
- Acetyl-CoA Carboxylase genetics, Acetyl-CoA Carboxylase metabolism, Adaptation, Physiological drug effects, Adaptation, Physiological genetics, Animals, Aspartate Aminotransferases genetics, Aspartate Aminotransferases metabolism, Dietary Proteins administration & dosage, Dietary Proteins pharmacology, Fatty Acid Synthases genetics, Fatty Acid Synthases metabolism, Gene Expression Regulation drug effects, Isocitrate Dehydrogenase genetics, Isocitrate Dehydrogenase metabolism, Liver drug effects, Liver enzymology, Liver metabolism, Malate Dehydrogenase genetics, Malate Dehydrogenase metabolism, Male, Reverse Transcriptase Polymerase Chain Reaction, Time Factors, Chickens genetics, Chickens metabolism, Lipid Metabolism genetics, Lipogenesis genetics
- Abstract
This experiment was conducted to determine possible relationships between certain indices of lipid metabolism and specific gene expression in chickens fed graded levels of dietary crude protein. Male, broiler chickens (Gallus gallus) growing from 7 to 28days of age were fed diets containing 12 or 30% protein ad libitum. Both groups were then switched to the diets containing the opposite level of protein. Birds were sampled at 0, 6, 9, 12, 18 and 24h following the switch in protein levels. Measurements taken included in vitro lipogenesis (IVL), malic enzyme (ME), aspartate aminotransferase (AAT) and isocitrate dehydrogenase (NADP) (ICD) activities. In addition, ME, AAT, ICD, fatty acid synthase (FAS), and acetyl coenzyme carboxylase (ACC) gene expression rates were determined. IVL and ME activities were inversely related to dietary protein levels (12 to 30%) and to acute changes from 12 to 30%. In contrast, expression of ME, FAS and ACC genes was decreased by feeding a 30% protein diet (acute or chronic feeding). Results of the present study demonstrate a continued role for protein in the regulation of broiler metabolism. It should be pointed out; however, that metabolic regulation at the gene level only occurs when feeding very high or very low levels of dietary protein., (Published by Elsevier Inc.)
- Published
- 2011
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