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Beta-adrenergic regulation of uncoupling protein expression in swine.
- Source :
-
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology [Comp Biochem Physiol A Mol Integr Physiol] 2007 Jun; Vol. 147 (2), pp. 395-403. Date of Electronic Publication: 2007 Jan 25. - Publication Year :
- 2007
-
Abstract
- This study examined the beta-adrenergic regulation of uncoupling protein (UCP) 2 and UCP3 gene expression in porcine tissues. In vitro experiments examined changes in UCP2 and UCP3 gene expression in middle (MSQ) and outer (OSQ) subcutaneous adipose tissues from crossbred neutered male pigs. Incubation of tissue slices (24 h) with 0 to 1000 nM isoproterenol increased UCP2 and UCP3 mRNA abundance in MSQ and OSQ, relative to 18S rRNA (P<0.05). For the in vivo experiment, nine randomly selected pigs (80 kg) were presented with a diet supplemented with 10.0 ppm ractopamine for 2 weeks. Another eight pigs were maintained on a control diet. Dietary ractopamine did not affect adipose UCP2 or UCP3 gene expression (P>0.05). However, UCP2 mRNA abundance was depressed in semitendinosus white (STW, P<0.05) and semitendinosus red (STR, P<0.001) by ractopamine feeding. Also, ractopamine decreased UCP3 mRNA abundance by 28% in STW (P<0.05). The in vitro data suggest that beta-adrenergic agonists directly affect adipose tissue UCP expression, although these adipose effects can be masked by the in vivo physiology. The in vivo data indicate that beta-adrenergic agonists may function in regulating UCP2 and UCP3 expression in selected muscles.
- Subjects :
- Animals
Ion Channels metabolism
Male
Mitochondrial Proteins metabolism
Phenethylamines pharmacology
RNA, Messenger genetics
RNA, Messenger metabolism
Swine
Uncoupling Protein 2
Uncoupling Protein 3
Weight Gain drug effects
Adrenergic beta-Agonists pharmacology
Gene Expression Regulation drug effects
Ion Channels genetics
Mitochondrial Proteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1095-6433
- Volume :
- 147
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
- Publication Type :
- Academic Journal
- Accession number :
- 17383207
- Full Text :
- https://doi.org/10.1016/j.cbpa.2007.01.007