1. Assessment of Interferon-Gamma Release Assay in Patients with Non-Tuberculous Mycobacteria Pulmonary Disease
- Author
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Qi Sun, Rongrong Wei, Guirong Wang, Song Zhipeng, Liping Pan, Hongyan Jia, Boping Du, Aiying Xing, Hongfei Duan, and Zongde Zhang
- Subjects
Adult ,Male ,medicine.medical_specialty ,Tuberculosis ,Interferon gamma release assay ,Mycobacterium Infections, Nontuberculous ,Disease ,Sensitivity and Specificity ,General Biochemistry, Genetics and Molecular Biology ,Mycobacterium tuberculosis ,Diagnosis, Differential ,Tuberculosis diagnosis ,Internal medicine ,Diabetes mellitus ,medicine ,Humans ,In patient ,Tuberculosis, Pulmonary ,Aged ,Retrospective Studies ,Aged, 80 and over ,biology ,business.industry ,Reproducibility of Results ,Retrospective cohort study ,Nontuberculous Mycobacteria ,Middle Aged ,bacterial infections and mycoses ,biology.organism_classification ,medicine.disease ,Female ,business ,Interferon-gamma Release Tests - Abstract
Background Interferon-gamma release assay (T-SPOT.TB) has the theoretical possibility of discriminating TB from most non-tuberculous mycobacteria (NTM) infections, but there are limited reports on the use of T-SPOT.TB for diseases due to NTM in high TB burden country. The aim of the present study was to assess the utility of T-SPOT.TB in patients with NTM pulmonary disease. Methods Clinical parameters and laboratory characteristics of patients with NTM pulmonary disease between July 2011 and Jan 2017 were investigated retrospectively and comprehensively reviewed. Results A total of 127 patients with NTM pulmonary disease were retrospectively reviewed. Seven NTM species were isolated from 115 patients, and the most common species were M. intracellulare (48.7%, 56/115) and M. abscessus (34.8%, 40/115). NTM isolates were mainly prevalent in people aged 50 years or older (73.0%). The overall positive rate of T-SPOT.TB test was 29.6% (24/81). In patients infected with NTM sharing the RD1 region of Mycobacterium tuberculosis (M. TB), 50% (3/6) were positive in the T-SPOT.TB test, whereas 28.0% (21/75) was positive in the group with NTM not sharing the RD1 region of M. TB. No significant difference was detected in the positive rate of T-SPOT.TB between definite (28.3%, 15/53) and probable disease (32.1%, 9/28). Conclusions Our data indicated a relatively high positive rate of T-SPOT.TB test in patients infected with NTM not sharing the RD1 region of M. TB. Thus, T-SPOT.TB test displays a limited ability in differentiating TB infection from NTM disease in a high TB burden country.
- Published
- 2019