Search

Resumo O objetivo deste experimento foi avaliar o efeito da época do ano sobre a biometria testicular e qualidade do sêmen fresco e congelado de touros Pantaneiros. Seis touros foram submetidos, mensalmente, durante um ano, à mensuração testicular e à coleta e congelamento do sêmen, utilizando-se meio à base de tris-gema-glicerol. O comprimento e o volume testiculares foram maiores (P < 0,05) nos meses de junho (11,1±0,8 cm; 652,7±138,0 cm3), julho (11,0±0,9 cm; 616,4±156,2 cm3) e setembro (11,8±0,8 cm; 692,4±141,3 cm3) que em outubro (9,4±1,2 cm; 498,5±154,3 cm3) e novembro (10,3±1,1cm; 560,0±111,8 cm3). A concentração espermática foi maior (P < 0,05) no mês de agosto (1265,0±436,8 x106sptz/mL) que nos meses de outubro, dezembro e janeiro (591,6±192,0; 627,5±314,4; 502,5±172,9 x106 sptz/mL, respectivamente). Não houve efeito da época do ano sobre a qualidade do sêmen fresco e congelado (motilidade, vigor, defeitos totais e integridade acrossomal) (P > 0,05). Conclui-se que touros Pantaneiros, na região do Distrito Federal, podem ser utilizados em programas de acasalamento natural em todas as épocas do ano. Visando ao enriquecimento do Banco Brasileiro de Germoplasma Animal com maior número de doses, sugere-se o congelamento do sêmen entre os meses de junho e agosto, embora este não apresente perda de qualidade nas diferentes épocas do ano. Abstract The purpose of this paper was to investigate the effect of the season of the year on testicular morphology, fresh and frozen/thawed semen quality from Pantaneiro bulls bred in Brasilia, DF. Six bulls were submitted, once a month for one year, to evaluation of testicular measurements, semen collection using an artificial vagina and cryopreserved with medium containing Tris-based, egg yolk and glycerol. The testicular length and volume were greater (P < 0.05) in June (11.1±0.8 cm; 652.7±138.0 cm3), July (11.0±0.9 cm; 616.4±156.2 cm3), and September (11.8±0.8 cm; 692.4±141.3 cm3) than October (9.4±1.2 cm; 498.5±154.3 cm3) and November (10.3±1.1 cm; 560.0±111.8 cm3). The sperm concentration was greater (P 0.05). In conclusion, Pantaneiro bulls, bred in the Midwest region of Brazil, can be used in natural mating programs in all seasons. Aiming to improve the Brazilian Animal Germplasm Bank with a higher number of samples collected, we suggest that the freezing of the semen is done between June and August, although there will be no losses of semen quality at any time.

Made available in DSpace on 2018-11-12T17:27:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-09-01. Added 1 bitstream(s) on 2018-11-12T17:35:25Z : No. of bitstreams: 1 S1809-68912015000300419.pdf: 632261 bytes, checksum: b1c1c8a5874371da4abc953c24a80830 (MD5) ResumoO exame ultrassonográfico é um meio de diagnóstico preciso para avaliação da gestação, além de ser totalmente inócuo para fêmea e fetos. Objetivou-se com este estudo determinar os valores de referência para a ecobiometria gestacional em cadelas de diferentes raças. Foram utilizadas 25 fêmeas multíparas, sendo cinco cadelas Bulldog inglês, cinco Pugs e 15 Shih Tzu, pesando de 4 a 25 kg e com idade entre 4 a 6 anos. As avaliações ecobiométricas como diâmetro da vesícula gestacional, comprimento femoral, espessura da placenta, diâmetro parietal, diâmetro e área abdominal, hepática e cardíaca foram realizadas durante as 2ª, 5ª, 6ª, 7ª e 8ª semanas gestacionais das cadelas. O estudo ecobiométrico em cadelas gestantes foi precocemente iniciado a partir da segunda semana gestacional. Mensurações pouco estudadas como o diâmetro e área hepática e valores ecobiométricos do coração fetal, podem fornecer informações úteis para a detecção precoce de anomalias congênitas que podem comprometer a viabilidade gestacional. Os resultados significativos (P < 0.001), obtidos para a biometria do diâmetro parietal (r2 = 81%) e abdominal (r2= 86%), área abdominal (r2 = 80%), comprimento do fêmur (r2 = 84%), comprimento (r2 = 79%), largura (r2 = 79%), área (r2 = 82%) e volume (r2 = 72%) cardíaco e área hepática (r2 = 71%) em conceptos braquiocefálicos, podem ajudar a avaliação do desenvolvimento dos fetos, complementando a ultrassonografia gestacional de cadelas e como modelo para o estudo em outras raças de cães e espécies animais. AbstractUltrasonography is an accurate pregnancy diagnostic method, besides being completely innocuous for female and fetuses evaluation. The objective of this paper was to determine the reference values for gestational echo biometry of different breeds of bitches. A total of 25 multiparous females were included in the experiment, five English Bulldog bitches, five Pugs and 15 Shih Tzu, weighing 4-25 kg and aged 4-6 years. The echo biometric assessments were performed during the 2nd, 5th, 6th, 7th and 8th weeks of pregnancy, including gestational vesicle diameter, femur length, placenta thickness, parietal diameter, liver, heart and abdominal diameter and area. Early echo biometric study started at the second week of gestation. Measurements like fetal heart and liver diameter and area are still poorly studied, but can provide useful information for early detection of congenital anomalies that may reduce the viability of pregnancy. The significant results (P < 0.001) obtained for biometrics (P < 0.001) of the parietal (r2 = 81%) and abdominal diameter (r2 = 86%), abdominal area (r2 = 80%), femur length (r2 = 84%), cardiac length (r2 = 79%), width (r2 = 79%), area (r2 = 82%) and volume (r2= 72%) and liver area (r2 = 71%) in brachycephalic conceptus may help to assess the development of fetuses, complementing the conventional gestational ultrasound of bitches and become a model for the study in other breeds of dogs and alternative animal species. Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias Universidade Federal Rural da Amazônia Universidade Estadual Paulista Faculdade de Ciências Agrárias e Veterinárias

The failure of passive immunity transfer, which has been a major obstacle in the sheep industry, is an important cause of neonatal diseases and mortality. In search of an effective management measure to solve this problem, the effects of the age and the monitoring of colostrum administration on the dynamics of the serum proteins were evaluated. Forty-four lambs were used and divided into two groups: monitored colostrums intake (MO), with 8 animals, and non-monitored colostrums intake (NM), with 36 animals, along with clinical evaluation, hemogram and total serum protein assessment and its electrophoretic fractioning, 24-72h after birth, at 7, 15, 30, 60 and 90 days of age. The MO group was followed in two additional times: before and six hours after colostrum intake. Total protein and gammaglobulin (g/dL) in the MO group presented the minimum values right after birth (3.62 ± 0.70 and 0.32 ± 0.12) and the maximum 24-72 hours after birth (7.98 ± 1.95 and 3.39 ± 1.13). Such concentrations also showed the same behavior in the NM group, with lower values (5.57 ± 1.45 and 1.80 ± 0.95), and with significant difference (p ≤ 0.05) between groups. Between 15 to 30 days, the values of these variables decreased in both groups, and increased up to 90 days. In all experimental moments, the MO group showed higher values of gammaglobulin than NM group. The conclusions of this paper are that most of protein fractions, mainly total protein and gammaglobulin, undergo changes with the age development, and that the monitoring of colostrum intake increased significantly the levels of gammaglobulin. KEYWORDS: Endogenous antibodies, failure of passive immunity transfer, immunoglobulins, neonatal period, serum proteins.

The present paper aimed at evaluating the effect of adding beneficial micro-organisms to the litters on litter quality, performance and carcass yield for broilers. A total of 240 one-day chicks were used, and randomly distributed in blocks with four treatments and four replications. The following treatments were carried out in the housing: Treatment 1 – Control with weekly spraying of water on the litters; Treatment 2 – Litter treated with a mixture of inoculated and fermented meal by micro-organisms and weekly spraying of water; Treatment 3 – Litter treated by weekly spraying of micro-organisms; Treatment 4 – Litter treated with the same mixture of meals from treatment two and weekly spraying of micro-organisms. Performance was evaluated by the feed consumption, weight gain, feed conversion, viability and carcass, breast and leg yield. From litter samples, pH, dry matter, ashes and nitrogen were evaluated. No differences were found among the treatments. In the conditions the animals were raised, it can be concluded that the treatment on the litter does not affect performance, carcass yield and quality of the litter for broilers. KEYWORDS: feed conversion; Gallus gallus ; litter pH; probiotic.

The aim of this paper was to evaluate microbiological characteristics of meat products produced under federal inspection by industries in the State of Parana (Brazil), following Brazilian regulations. According to the type of meat product, quantification of nitrite and starch was also determined. All samples were collected at the plant and all analysis were carried out according to the Brazilian Ministry of Agriculture, Livestock and Food Supply´s recommendations. From 131 samples analyzed, five (3.82%) were contaminated by Salmonella sp. and three (2.3%) presented fecal coliforms. The other samples were legally acceptable. Frankfurters showed starch levels ranging from 1.71% to 10.28%. In bologna, this variation ranged from 3.01% to 16.5%. Residual nitrite levels in all products ranged from 3.7 mg/kg to 293.10 mg/kg. An amount of 23 samples (18.4%) had residual nitrite levels higher than legal limits, with a medium level of 395.91 mg/kg. The results found suggest that, although the analyzed samples present good sanitary quality, some producers need to be more carefully inspected, in order to attend Brazilian regulations for meat products. KEYWORDS: inspection; meat; meat products; nitrite; quality; starch.

The activity of the insecticide thiamethoxam on populations of Musca domestica was assessed through in vitro tests, controlled environment and field conditions. The flies were gathered from a colony maintained by the Laboratory of Parasitic Diseases, Department of Epidemiology and Public Health, Universidade Federal Rural do Rio de Janeiro, and from the landfill of the city of Seropedica-RJ, Brazil. Regarding the in vitro assay, thiamethoxan was previously diluted in acetone, resulting in concentrations of 5, 7.5, 10, 12.5, 15, 17.5, 20, 22.5, 25, 27, 5 and 30 mg/ml. Then, filter paper discs were impregnated with 1 ml of each of these dilutions. Thus, the test was conducted with adult flies maintained in Petri dishes. For the controlled environment and field conditions, trial baits were used with the active or a placebo (control group). The lethal concentration 50 (LC50) of thiamethoxam on M. domestica found in the in vitro assay was 0.2612 mg / cm ². The efficacy of thiamethoxan baits on the controlled environment and field conditions trials was 94.06 % and 98.09 %, respectively. KEYWORDS: Baits, Musca domestica, neonicotinoids, thiamethoxam.

The present work aimed to investigate, in vitro, the efficacy of the honey of Tetragonisca angustula, against the microorganisms found in the auditory canal inflammations of dogs. The samples were collected through a sterile swab of secretion of the auditory canal of dogs with clinical otitis. The material was fixed in glass slide by performing the smear, Gram stained, and submitted to microscopic identification. The samples were inoculated in Tryptone Soya Broth culture media and incubated at 37°C for 24 hours. After verifying that there was growth, the samples were cultured in Petri’s plates containing Chapman, Levine and Mueller-Hinton, and incubated at 37°C for 24 hours. Filter paper discs were adapted, sterilized in autoclave and embedded in honey for the antibiogram. The disks impregnated with honey were placed in Petri’s plates containing the contamined material and again incubated at 37°C for 24 hours. This experiment verified the formation of inhibition halos in the antibiograma with the honey of the Tetragonisca angustula, showing to be effective in the control of Staphylococcus sp, Bacillus sp and yeasts. KEYWORDS: Honey, otitis, treatment, Tetragonisca angustula.

The distal third of the radius and ulna is the most prevalent site in traumatic processes of thoracic limbs in dogs. Especially in miniature breeds, fractures on the distal third of the radius and ulna are frequently associated with complications such as lack of bone union or delayed union at the fracture focus. The purpose of this paper was to assess the use of semitubular titanium plates with clamps specially developed for treating radial and ulnar fractures in miniature dog breeds. In a 14-month period, 15 animals were seen with radial and ulnar fracture, which had had a plate implant and afterwards were assessed by means of a clinical and radiological examination every 30 days. The animals recovered the function of their operated limbs between 4 and 7 days after the surgery. By X-ray examination, bone consolidation was observed in all dogs in less than 90 days. Post-op complications occurred in two cases: rotation of the distal bone fragment after loss of function of distal screws in one dog, and in another, exposure of distal end of plate, which had to be removed. The use of the plate with clamps is an effective technique to correct this type of fracture. KEYWORDS: Dog, ortopaedic, osteosynthesis, semitubular plates with clamps, semitubular titanium plates with clamps.