1. Suppressive effect of herbal compound 861 on chemical hepatocarcinogenesis in rats.
- Author
-
Cui R, Wang B, Zhang F, Chen G, and Yin S
- Subjects
- 2-Acetylaminofluorene, Animals, Blotting, Northern, Blotting, Western, Body Weight, Diethylnitrosamine, Female, Glutathione Transferase genetics, Liver pathology, Liver Neoplasms, Experimental chemically induced, Liver Neoplasms, Experimental pathology, Organ Size, Rats, Rats, Sprague-Dawley, Drugs, Chinese Herbal therapeutic use, Liver Neoplasms, Experimental drug therapy
- Abstract
Objective: To investigate the effects of herbal compound 861 (Cpd861) on hepatocarcinogenesis induced by diethylntrosamine and 2-acetylaminofluorene (DEN-AAF) in female Sprague Dawley rats., Methods: Liver preneoplastic foci were induced using the DEN-AAF method in female Sprague Dawley rats, which were then treated with Cpd861. For quantitative assessment of liver preneoplastic foci, the placental form of glutathione-S-transferase (GST-P) positive foci were measured using immunohistochemical staining and image analysis. GST-P protein expression was measured by Western blotting, mRNA expression was assessed by Northern blotting., Results: Treatment using DEN-AAF caused a significant decrease in body weight and increase in liver weight compared to the control group. Oral Cpd861 administration essentially prevented DEN-AAF-induced body weight loss and liver weight increase. When 2-AAF was followed by treatment with Cpd861, there was a decrease in the number of large foci as compared to 2-AAF alone. However, there were still considerable numbers of small mixed clear/vacuolated cell foci, some of which were positive for GST-P. Significant increase in GST-P protein and mRNA expression were observed in the DEN-AAF group, while treatment with Cpd861 inhibited the increase. The effect of Cpd861 on hepatocarcinogenesis occurred in a concentration-dependent manner., Conclusion: Chinese herbal compound Cpd861 prevents hepatocarcinogenesis in DEN-AAF-induced liver preneoplastic lesions in rats.
- Published
- 2003