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Suppressive effect of herbal compound 861 on chemical hepatocarcinogenesis in rats.
- Source :
-
Chinese medical journal [Chin Med J (Engl)] 2003 Mar; Vol. 116 (3), pp. 378-82. - Publication Year :
- 2003
-
Abstract
- Objective: To investigate the effects of herbal compound 861 (Cpd861) on hepatocarcinogenesis induced by diethylntrosamine and 2-acetylaminofluorene (DEN-AAF) in female Sprague Dawley rats.<br />Methods: Liver preneoplastic foci were induced using the DEN-AAF method in female Sprague Dawley rats, which were then treated with Cpd861. For quantitative assessment of liver preneoplastic foci, the placental form of glutathione-S-transferase (GST-P) positive foci were measured using immunohistochemical staining and image analysis. GST-P protein expression was measured by Western blotting, mRNA expression was assessed by Northern blotting.<br />Results: Treatment using DEN-AAF caused a significant decrease in body weight and increase in liver weight compared to the control group. Oral Cpd861 administration essentially prevented DEN-AAF-induced body weight loss and liver weight increase. When 2-AAF was followed by treatment with Cpd861, there was a decrease in the number of large foci as compared to 2-AAF alone. However, there were still considerable numbers of small mixed clear/vacuolated cell foci, some of which were positive for GST-P. Significant increase in GST-P protein and mRNA expression were observed in the DEN-AAF group, while treatment with Cpd861 inhibited the increase. The effect of Cpd861 on hepatocarcinogenesis occurred in a concentration-dependent manner.<br />Conclusion: Chinese herbal compound Cpd861 prevents hepatocarcinogenesis in DEN-AAF-induced liver preneoplastic lesions in rats.
- Subjects :
- 2-Acetylaminofluorene
Animals
Blotting, Northern
Blotting, Western
Body Weight
Diethylnitrosamine
Female
Glutathione Transferase genetics
Liver pathology
Liver Neoplasms, Experimental chemically induced
Liver Neoplasms, Experimental pathology
Organ Size
Rats
Rats, Sprague-Dawley
Drugs, Chinese Herbal therapeutic use
Liver Neoplasms, Experimental drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 0366-6999
- Volume :
- 116
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Chinese medical journal
- Publication Type :
- Academic Journal
- Accession number :
- 12781041