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Objective To investigate the clinical effect and safety of zoned low negative pressure liposuction combined with low energy bipolar radiofrequency and granular fat filling in mid-lower facial rejuvenation. Methods From October 2019 to April 2023, 32 patients (64 sides) with nasolabial depression with mid-lower facial fat accumulation and skin relaxation were retrospectively analyzed. The patients were followed up for 6 months after receiving bucaal low negative pressure liposuction combined with low energy bipolar radio frequency, nasolabial groove and lateral buccal depression granule fat filling treatment. The postoperative effect was evaluated by doctors and patients respectively, and the postoperative complications were counted. Results Among the 32 patients, the mean body mass index was (22.2±1.6) kg/m2, the mean fat extracted per zone was (3.6±2.4) ml, and the mean radiofrequency energy per zone was (1.6±0.5) kJ. After 6 months of follow-up, the patient's facial contour was smooth, skin elasticity was enhanced, and wrinkles were reduced. The average score on the 6-point grading scale (GGS) was decreased from (3.5±0.7) to (1.4±0.8) (P<0.05), the average facial laxity rating scale (FLR) score decreased from (5.3±0.6) points to (2.4±0.5) points (P<0.05), the self-rated satisfaction rate of patients was 90.6%. Postoperative Ⅰ degree skin scald occurred in 1 case, and oral Angle was slightly skewed in 2 cases in the early stage after surgery. The symptoms disappeared completely after 3 to 12 weeks of conservative treatment, and no complications occurred in the remaining patients. Conclusion The low negative pressure liposuction combined with low energy bipolar radiofrequency and particle fat filling can effectively improve the fat accumulation in the cheek, the aging of the "law line" in the nasolabial groove and the loose sag in the mid-lower face, which is a safe and reliable treatment method worthy of promotion. [ABSTRACT FROM AUTHOR]

Objective To explore the effect of interleukin-35 (IL-35) on osteogenic differentiation of dental pulp stem cells (DPSCs). Methods The DPSCs were isolated from pulp tissue of orthodontic patients and cultured in osteogenic induction medium for 21 days. The osteogenic related genes such as runt-related transcription factors (RUNX2) and collagen type 1 (COL-1) and the expression of receptors of IL-35 (glycoprotein 130, gp130) were detected by RT-qPCR. The calcium mineralized nodules were stained by alizarin red and the staining was quantitative analyzed by cetylpyridinium chloride (CPC). The osteogenic differentiation of DPSCs was stimulated by IL-35 with the concentration of 100 ng/ml. Under the condition with or without IL-35, the expression of osteogenic related gene and the quantitative analysis results of alizarin red staining were compared. Results The osteogenic differentiation of DPSCs was demonstrated by alizarinred staining and expression of osteogenic related genes (P<0.05). The differentiation process accompanied with increased expression of gp130 (P< 0.05). Under the action of human recombinant IL-35, the DPSCs showed strong alizarin red staining (P<0.001) and accompanied with increased expression of RUNX2 and COL-1 (P<0.05). Conclusion The IL-35 can suppress inflammation and promote the osteogenic differentiation of DPSCs. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of peroxisome proliferator-activated receptor gamma (PPARγ) gene on the migration of adipose-derived stem cells (ADSCs) and to explore the possible signaling pathways. Methods Human ADSCs were extracted and cultured in vitro, and different concentrations of PPARγinhibitor bisphenol a diglycidyl ether (BADGE) were applied to ADSCs. The migration of ADSCs was observed by scratch test and Transwell chamber experiment. PCR and western blot were used to detect the expression of MMP2, MMP9, CXCR4 and related proteins. Results The migration ability of ADSCs decreased in both 2D and 3D space (P<0.05). The migration of ADSCs in 2D space: at 72 h, the migration rate of control group was about 63%, and that of 25 μm/L group was about 43%, 50 μm /L group was approximately 24%. Three-dimensional space: the number of ADSCs transfected into the chamber was decreased by 14% and 28%, respectively, compared with the control group by inhibiting the PPARγgene at 24 h in 25 μm/L and 50 μm/L group (P<0.05). The expression of MMP2, MMP9, CXCR4 and related proteins were significantly decreased after inhibition of PPARγgene (P<0.05). Conclusion The migration ability of ADSCs after inhibition of PPARγgene was decreased. The inhibitory effect increased with the increase of inhibitor concentration, and the possible regulatory mechanism was to inhibit the transmission of MMP and CXCR4 signaling pathways. [ABSTRACT FROM AUTHOR]

Objective To explore the characteristics, mechanism and surgical treatment of prepubertal gynecomastia (PPGM). Methods The clinical data of 9 PPGM patients underwent surgical treatment from 2003 to 2018 were collected and retrospectively analyzed. Seven patients underwent the liposuction and mammary gland excision through an incision along the lower edge of mammary areola, 1 patient underwent the mammary gland excision through an incision along the lower edge of areola and 1 patient underwent the unilateral mammary gland excision through an incision along the lower edge of areola, liposuction and fat grafting of contralateral breast. The excised tissues were sent to pathological examination. Results No complications such as infection and hematoma occurred. The pathological examination results were consistent with the diagnosis of PPGM. No recurrence was found during the follow up from 1 to 16 years. Conclusion PPGM is a rare type of gynecomastia. The surgical method should be reasonably selected based on the clear etiological diagnosis and drug control. For patients with obvious gland development and psychiatric symptoms, surgical treatment of PPGM can achieve satisfactory efficacy. [ABSTRACT FROM AUTHOR]

Objective To explore the application of modified orbital fat-releasing lower blepharoplasty in the treatment of lower eyelid depression with lacrimal sulcus deformity. Methods From October 2016 to 2017, 20 patients with lower eyelid depression and lacrimal sulcus deformity who underwent blepharoplasty were selected as study subjects. Modified orbital fat-releasing lower blepharoplasty was selected as the treatment option, and limited orbital fat was precisely fixed to the superficial layer tissue of the severe lacrimal sulcus deformity. Results All patients were followed up from 6 to 24 months. Postoperative efficacy was good and patients were satisfied with the outcome. No complications occurred. Conclusion The application of modified orbital fat-releasing lower blepharoplasty in the treatment of lower eyelid depression with lacrimal sulcus deformity can achieve satisfactory results. Postoperatively, patients were able to achieve a more natural appearance. [ABSTRACT FROM AUTHOR]