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Your search keyword '"Masayuki Endo"' showing total 3 results
Start Over Author "Masayuki Endo" Topic nanostructures Publication Year Range Last 50 years Journal chemical communications
3 results on '"Masayuki Endo"'

1. Construction of an optically controllable CRISPR-Cas9 system using a DNA origami nanostructure

Author

Hiroshi Sugiyama, Katsuhiko Abe, and Masayuki Endo

Subjects
Nanostructure, Cleavage (embryo), Catalysis, immune system diseases, CRISPR-Associated Protein 9, Materials Chemistry, DNA origami, CRISPR, A-DNA, Cloning, Molecular, DNA Cleavage, skin and connective tissue diseases, Nuclease, biology, Cas9, Chemistry, Metals and Alloys, DNA, General Chemistry, Photochemical Processes, Nanostructures, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Enzyme Activation, Ceramics and Composites, biology.protein, Biophysics, CRISPR-Cas Systems
Abstract

We demonstrated the photo-controlled sequence-selective dsDNA cleavage using a DNA origami structure with Cas9 nuclease. The activity of Cas9 incorporated inside the ring-shaped DNA origami was completely suppressed. After photoirradiation, Cas9 was released, and its activity for selective dsDNA cleavage was restored.

Published
2021

2. A lock-and-key mechanism for the controllable fabrication of DNA origami structures

Author

Hiroshi Sugiyama, Kumi Hidaka, Atsushi Maruyama, Arivazhagan Rajendran, Naohiko Shimada, and Masayuki Endo

Subjects
chemistry.chemical_classification, Nanostructure, Fabrication, Materials science, Polymers, Metals and Alloys, Nanotechnology, DNA, General Chemistry, DNA chemical synthesis, Polymer, Catalysis, Nanostructures, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Mechanism (engineering), Folding (chemistry), chemistry, Materials Chemistry, Ceramics and Composites, Nucleic Acid Conformation, DNA origami, Sulfonic Acids
Abstract

Controllable fabrication of DNA origami structures was achieved using cationic comb-type copolymers (CCCs) as locks and polyvinyl sulphonic acid (PVS) as a key. A CCC binds to the phosphate backbone of either M13mp18/staples alone or both together and restricts origami folding, while PVS unlocks the CCC, restoring the formation of origami structures.

Published
2014

3. A lock-and-key mechanism for the controllable fabrication of DNA origami structures.

Author

Arivazhagan Rajendran, Kumi Hidaka, Hiroshi Sugiyama, Masayuki Endo, Naohiko Shimada, and Atsushi Maruyama

Subjects
DNA folding, POLYMER research, NANOSTRUCTURES, CATIONIC polymers, ATOMIC force microscopy
Abstract

Controllable fabrication of DNA origami structures was achieved using cationic comb-type copolymers (CCCs) as locks and polyvinyl sulphonic acid (PVS) as a key. A CCC binds to the phosphate backbone of either M13mp18/staples alone or both together and restricts origami folding, while PVS unlocks the CCC, restoring the formation of origami structures. [ABSTRACT FROM AUTHOR]

Published
2014
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