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Construction of an optically controllable CRISPR-Cas9 system using a DNA origami nanostructure
- Source :
- Chemical Communications. 57:5594-5596
- Publication Year :
- 2021
- Publisher :
- Royal Society of Chemistry (RSC), 2021.
-
Abstract
- We demonstrated the photo-controlled sequence-selective dsDNA cleavage using a DNA origami structure with Cas9 nuclease. The activity of Cas9 incorporated inside the ring-shaped DNA origami was completely suppressed. After photoirradiation, Cas9 was released, and its activity for selective dsDNA cleavage was restored.
- Subjects :
- Nanostructure
Cleavage (embryo)
Catalysis
immune system diseases
CRISPR-Associated Protein 9
Materials Chemistry
DNA origami
CRISPR
A-DNA
Cloning, Molecular
DNA Cleavage
skin and connective tissue diseases
Nuclease
biology
Cas9
Chemistry
Metals and Alloys
DNA
General Chemistry
Photochemical Processes
Nanostructures
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Enzyme Activation
Ceramics and Composites
biology.protein
Biophysics
CRISPR-Cas Systems
Subjects
Details
- ISSN :
- 1364548X and 13597345
- Volume :
- 57
- Database :
- OpenAIRE
- Journal :
- Chemical Communications
- Accession number :
- edsair.doi.dedup.....761481922ae7adb3bf02cba4cfac0455