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Your search keyword '"Masayuki Endo"' showing total 1 results
Start Over Author "Masayuki Endo" Publication Year Range Last 10 years Journal chemical communications

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1 results on '"Masayuki Endo"'

1. Construction of an optically controllable CRISPR-Cas9 system using a DNA origami nanostructure

Author

Hiroshi Sugiyama, Katsuhiko Abe, and Masayuki Endo

Subjects
Nanostructure, Cleavage (embryo), Catalysis, immune system diseases, CRISPR-Associated Protein 9, Materials Chemistry, DNA origami, CRISPR, A-DNA, Cloning, Molecular, DNA Cleavage, skin and connective tissue diseases, Nuclease, biology, Cas9, Chemistry, Metals and Alloys, DNA, General Chemistry, Photochemical Processes, Nanostructures, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Enzyme Activation, Ceramics and Composites, biology.protein, Biophysics, CRISPR-Cas Systems
Abstract

We demonstrated the photo-controlled sequence-selective dsDNA cleavage using a DNA origami structure with Cas9 nuclease. The activity of Cas9 incorporated inside the ring-shaped DNA origami was completely suppressed. After photoirradiation, Cas9 was released, and its activity for selective dsDNA cleavage was restored.

Published
2021

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