1. Modulation of TCRβ surface expression during TCR revision.
- Author
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Simmons KB, Wubeshet M, Ames KT, McMahan CJ, Hale JS, and Fink PJ
- Subjects
- Animals, CD4-Positive T-Lymphocytes metabolism, Down-Regulation genetics, Gene Expression genetics, Immune Tolerance genetics, Mice, Mice, Transgenic, Receptors, Antigen, T-Cell, alpha-beta metabolism, Transgenes genetics, Gene Rearrangement, beta-Chain T-Cell Antigen Receptor, Receptors, Antigen, T-Cell, alpha-beta biosynthesis, Receptors, Antigen, T-Cell, alpha-beta genetics
- Abstract
TCR revision is a tolerance mechanism by which self-reactive TCRs expressed by mature CD4(+) peripheral T cells are replaced by receptors encoded by genes generated by post-thymic DNA rearrangement. The downmodulation of surface TCR expression initiates TCR revision, and serves as a likely trigger for the induction of the recombinase machinery. We show here in a Vβ5 transgenic mouse model system that downregulation of the self-reactive transgene-encoded TCR is not maintained by transgene loss or diminished transcription or translation. The downregulation of surface TCR expression likely occurs in two stages, only one of which requires tolerogen expression., (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Published
- 2012
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