1. Liraglutide attenuates high glucose-induced abnormal cell migration, proliferation, and apoptosis of vascular smooth muscle cells by activating the GLP-1 receptor, and inhibiting ERK1/2 and PI3K/Akt signaling pathways
- Author
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Lili Shi, Li-Hong Zhou, Xiao-Min Liu, Ye Ji, Yanbo Li, Ying Xu, Xiaoyan Jiang, Wei Jiang, and Ping Meng
- Subjects
Male ,medicine.medical_specialty ,Vascular smooth muscle ,Proto-Oncogene Proteins c-akt ,MAP Kinase Signaling System ,Endocrinology, Diabetes and Metabolism ,Myocytes, Smooth Muscle ,Apoptosis ,Pharmacology ,Glucagon-Like Peptide-1 Receptor ,Muscle, Smooth, Vascular ,Rats, Sprague-Dawley ,Phosphatidylinositol 3-Kinases ,Cell Movement ,Internal medicine ,Vascular smooth muscle cells ,Medicine ,Myocyte ,Animals ,Hypoglycemic Agents ,Protein kinase B ,Glucagon-like peptide 1 receptor ,Cells, Cultured ,Cell Proliferation ,Phosphoinositide-3 Kinase Inhibitors ,Original Investigation ,ERK1/2 ,business.industry ,Cell growth ,Liraglutide ,Akt ,Rats ,Endocrinology ,Glucose ,Glucagon-like peptide receptor ,High glucose ,Cardiology and Cardiovascular Medicine ,business ,medicine.drug - Abstract
Background As a new anti-diabetic medicine, Liraglutide (LIRA), one of GLP-1 analogues, has been found to have an anti-atherosclerotic effect. Since vascular smooth muscle cells (VSMCs) play pivotal roles in the occurrence of diabetic atherosclerosis, it is important to investigate the role of LIRA in reducing the harmful effects of high-glucose (HG) treatment in cultured VSMCs, and identifying associated molecular mechanisms. Methods Primary rat VSMCs were exposed to low or high glucose-containing medium with or without LIRA. They were challenged with HG in the presence of phosphatidylinositol 3-kinase (PI3K), extracellular signal-regulated kinase (ERK)1/2, or glucagon-like peptide receptor (GLP-1R) inhibitors. Cell proliferation and viability was evaluated using a Cell Counting Kit-8. Cell migration was determined by Transwell migration and scratch wound assays. Flow cytometry and Western blotting were used to determine apoptosis and protein expression, respectively. Results Under the HG treatment, VSMCs exhibited increased migration, proliferation, and phosphorylation of protein kinase B (Akt) and ERK1/2, along with reduced apoptosis (all p
- Published
- 2014