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Liraglutide attenuates high glucose-induced abnormal cell migration, proliferation, and apoptosis of vascular smooth muscle cells by activating the GLP-1 receptor, and inhibiting ERK1/2 and PI3K/Akt signaling pathways
- Source :
- Cardiovascular Diabetology
- Publication Year :
- 2014
-
Abstract
- Background As a new anti-diabetic medicine, Liraglutide (LIRA), one of GLP-1 analogues, has been found to have an anti-atherosclerotic effect. Since vascular smooth muscle cells (VSMCs) play pivotal roles in the occurrence of diabetic atherosclerosis, it is important to investigate the role of LIRA in reducing the harmful effects of high-glucose (HG) treatment in cultured VSMCs, and identifying associated molecular mechanisms. Methods Primary rat VSMCs were exposed to low or high glucose-containing medium with or without LIRA. They were challenged with HG in the presence of phosphatidylinositol 3-kinase (PI3K), extracellular signal-regulated kinase (ERK)1/2, or glucagon-like peptide receptor (GLP-1R) inhibitors. Cell proliferation and viability was evaluated using a Cell Counting Kit-8. Cell migration was determined by Transwell migration and scratch wound assays. Flow cytometry and Western blotting were used to determine apoptosis and protein expression, respectively. Results Under the HG treatment, VSMCs exhibited increased migration, proliferation, and phosphorylation of protein kinase B (Akt) and ERK1/2, along with reduced apoptosis (all p
- Subjects :
- Male
medicine.medical_specialty
Vascular smooth muscle
Proto-Oncogene Proteins c-akt
MAP Kinase Signaling System
Endocrinology, Diabetes and Metabolism
Myocytes, Smooth Muscle
Apoptosis
Pharmacology
Glucagon-Like Peptide-1 Receptor
Muscle, Smooth, Vascular
Rats, Sprague-Dawley
Phosphatidylinositol 3-Kinases
Cell Movement
Internal medicine
Vascular smooth muscle cells
Medicine
Myocyte
Animals
Hypoglycemic Agents
Protein kinase B
Glucagon-like peptide 1 receptor
Cells, Cultured
Cell Proliferation
Phosphoinositide-3 Kinase Inhibitors
Original Investigation
ERK1/2
business.industry
Cell growth
Liraglutide
Akt
Rats
Endocrinology
Glucose
Glucagon-like peptide receptor
High glucose
Cardiology and Cardiovascular Medicine
business
medicine.drug
Subjects
Details
- ISSN :
- 14752840
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Cardiovascular diabetology
- Accession number :
- edsair.doi.dedup.....8b3c766815abeb35941cafd240829e28