1. The T-lineage-affiliated CD2 gene lies within an open chromatin environment in acute promyelocytic leukemia cells.
- Author
-
Grimwade D, Outram SV, Flora R, Ings SJ, Pizzey AR, Morilla R, Craddock CF, Linch DC, and Solomon E
- Subjects
- Cell Lineage, Chromatin chemistry, Chromatin genetics, Deoxyribonuclease I metabolism, Humans, Immunophenotyping, Jurkat Cells, Leukemia, Promyelocytic, Acute pathology, Polymerase Chain Reaction methods, T-Lymphocytes cytology, CD2 Antigens genetics, Chromatin physiology, Leukemia, Promyelocytic, Acute genetics, T-Lymphocytes physiology
- Abstract
The nature of hemopoietic progenitors subject to leukemic transformation in acute myeloid leukemia (AML) has not been clearly defined. To address this issue, we have used DNase I hypersensitivity assays to study the chromatin structure surrounding the T-lineage-affiliated CD2 gene in the acute promyelocytic subtype of AML (APL). Upstream and downstream flanking regions of CD2 were found to be hypersensitive to DNase I in primary APL blasts, with an identical pattern of hypersensitive sites to those detected in cells of T-lineage. All of the sites were confirmed to be inaccessible to DNase I in B-lineage leukemia cells. The demonstration of T-cell-associated chromatin features in primary APL blasts has implications for the origin of APL that may arise in more primitive progenitors than previously considered to be the case.
- Published
- 2002