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The T-lineage-affiliated CD2 gene lies within an open chromatin environment in acute promyelocytic leukemia cells.
- Source :
-
Cancer research [Cancer Res] 2002 Aug 15; Vol. 62 (16), pp. 4730-5. - Publication Year :
- 2002
-
Abstract
- The nature of hemopoietic progenitors subject to leukemic transformation in acute myeloid leukemia (AML) has not been clearly defined. To address this issue, we have used DNase I hypersensitivity assays to study the chromatin structure surrounding the T-lineage-affiliated CD2 gene in the acute promyelocytic subtype of AML (APL). Upstream and downstream flanking regions of CD2 were found to be hypersensitive to DNase I in primary APL blasts, with an identical pattern of hypersensitive sites to those detected in cells of T-lineage. All of the sites were confirmed to be inaccessible to DNase I in B-lineage leukemia cells. The demonstration of T-cell-associated chromatin features in primary APL blasts has implications for the origin of APL that may arise in more primitive progenitors than previously considered to be the case.
- Subjects :
- Cell Lineage
Chromatin chemistry
Chromatin genetics
Deoxyribonuclease I metabolism
Humans
Immunophenotyping
Jurkat Cells
Leukemia, Promyelocytic, Acute pathology
Polymerase Chain Reaction methods
T-Lymphocytes cytology
CD2 Antigens genetics
Chromatin physiology
Leukemia, Promyelocytic, Acute genetics
T-Lymphocytes physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0008-5472
- Volume :
- 62
- Issue :
- 16
- Database :
- MEDLINE
- Journal :
- Cancer research
- Publication Type :
- Academic Journal
- Accession number :
- 12183432