1. Enhancement of rat hepatic macrophages by treatment with interleukin-2 and streptococcal preparation OK432, with reference to antitumor activity, soluble factor production and Ia expression
- Author
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Tetsu Sasaoki, Naomi Funaki, Shigeyuki Itai, Kazunobu Monden, Hiroaki Higashituji, Shigeki Arii, Takayoshi Tobe, and Yukito Adachi
- Subjects
Cytotoxicity, Immunologic ,Male ,Interleukin 2 ,Cancer Research ,medicine.medical_specialty ,medicine.medical_treatment ,Immunology ,Pharmacology ,Biology ,Picibanil ,Superoxides ,In vivo ,Internal medicine ,Cell Adhesion ,Tumor Cells, Cultured ,medicine ,Animals ,Humans ,Immunologic Factors ,Immunology and Allergy ,Macrophage ,Carbon Radioisotopes ,Cytotoxicity ,Tumor Necrosis Factor-alpha ,Macrophages ,Histocompatibility Antigens Class II ,Rats, Inbred Strains ,Neoplasms, Experimental ,Cytostasis ,In vitro ,Rats ,Cytokine ,Endocrinology ,Liver ,Oncology ,Autoradiography ,Interleukin-2 ,Tumor necrosis factor alpha ,medicine.drug - Abstract
The effect of biological response modifiers, such as interleukin-2 (IL-2) and streptococcal preparation OK432, on the functions of hepatic macrophages was investigated. The macrophages, even with no exogenous stimulation, produced superoxide anion (O2-) and tumor necrosis factor (TNF), displayed cytotoxicity against K562 cells and cytostasis against P815 cells and expressed immune-region-associated antigen (Ia). IL-2 administered in vitro or in vivo enhanced O2- production by hepatic macrophages and the intravenous injection of OK432 also enhanced O2- production. Furthermore, IL-2 added to the culture medium of hepatic macrophages isolated from OK432-injected rats augmented O2- production even more. The TNF production and Ia expression of the macrophages were also increased by the intravenous injection of OK432. As with O2- production, the cytotoxicity of the cells was enhanced by OK432 injection or by IL-2 added to the culture medium and the combination of OK432 and IL-2 augmented their cytotoxicity even more. Thus, the present study suggested that IL-2 and OK432 induce the augmentation of the antitumor activity of hepatic macrophages, partly as a result of the increase in production of O2- and TNF and Ia expression.
- Published
- 1992