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Enhancement of rat hepatic macrophages by treatment with interleukin-2 and streptococcal preparation OK432, with reference to antitumor activity, soluble factor production and Ia expression
- Source :
- Cancer Immunology Immunotherapy. 35:75-82
- Publication Year :
- 1992
- Publisher :
- Springer Science and Business Media LLC, 1992.
-
Abstract
- The effect of biological response modifiers, such as interleukin-2 (IL-2) and streptococcal preparation OK432, on the functions of hepatic macrophages was investigated. The macrophages, even with no exogenous stimulation, produced superoxide anion (O2-) and tumor necrosis factor (TNF), displayed cytotoxicity against K562 cells and cytostasis against P815 cells and expressed immune-region-associated antigen (Ia). IL-2 administered in vitro or in vivo enhanced O2- production by hepatic macrophages and the intravenous injection of OK432 also enhanced O2- production. Furthermore, IL-2 added to the culture medium of hepatic macrophages isolated from OK432-injected rats augmented O2- production even more. The TNF production and Ia expression of the macrophages were also increased by the intravenous injection of OK432. As with O2- production, the cytotoxicity of the cells was enhanced by OK432 injection or by IL-2 added to the culture medium and the combination of OK432 and IL-2 augmented their cytotoxicity even more. Thus, the present study suggested that IL-2 and OK432 induce the augmentation of the antitumor activity of hepatic macrophages, partly as a result of the increase in production of O2- and TNF and Ia expression.
- Subjects :
- Cytotoxicity, Immunologic
Male
Interleukin 2
Cancer Research
medicine.medical_specialty
medicine.medical_treatment
Immunology
Pharmacology
Biology
Picibanil
Superoxides
In vivo
Internal medicine
Cell Adhesion
Tumor Cells, Cultured
medicine
Animals
Humans
Immunologic Factors
Immunology and Allergy
Macrophage
Carbon Radioisotopes
Cytotoxicity
Tumor Necrosis Factor-alpha
Macrophages
Histocompatibility Antigens Class II
Rats, Inbred Strains
Neoplasms, Experimental
Cytostasis
In vitro
Rats
Cytokine
Endocrinology
Liver
Oncology
Autoradiography
Interleukin-2
Tumor necrosis factor alpha
medicine.drug
Subjects
Details
- ISSN :
- 14320851 and 03407004
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Cancer Immunology Immunotherapy
- Accession number :
- edsair.doi.dedup.....f269edab87b9f7cfec060acca64f691c