1. Co-expression of glial fabrillary acidic protein and vimentin in reactive astrocytes following brain injury in rats
- Author
-
J. Boya, A.L. Carbonell, and Calvo Jl
- Subjects
Pathology ,medicine.medical_specialty ,Central nervous system ,Vimentin ,macromolecular substances ,Glial Fibrillary Acidic Protein ,medicine ,Animals ,Molecular Biology ,Cerebral Cortex ,biology ,Glial fibrillary acidic protein ,General Neuroscience ,Rats, Inbred Strains ,GFAP stain ,Immunohistochemistry ,Rats ,medicine.anatomical_structure ,nervous system ,Cerebral cortex ,Astrocytes ,Brain Injuries ,Immunology ,biology.protein ,Neuroglia ,Neurology (clinical) ,Developmental Biology ,Astrocyte - Abstract
The immunohistochemical expression of glial fibrillary acidic protein (GFAP) and vimentin (VIM) was studied in reactive astrocytes of the rat cerebral cortex 5 days after a brain injury. Seriated Epon semithin sections were immunostained alternatively for GFAP or VIM. Thereafter, both antigens were detected in consecutive sections of the same cell. Bordering the wound, an inner reactive glial layer 300–350 μm thick, showed positive astrocytes with the two immunohistochemical techniques. In this layer, about 60% of the GFAP positive astrocytes were also positive for VIM. Outside the inner layer, only GFAP positive astrocytes could be found.
- Published
- 1991