1. Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells
- Author
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Pierre Layrolle, Nathalie Gallay, Dominique Kerboeuf, Jochen Ringe, Philippe Rosset, Luc Sensebé, Pierre Charbord, Bruno Delorme, Christian Jorgensen, Yves Le Vern, Thomas Häupl, Institut National de la Santé et de la Recherche Médicale (INSERM), Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), U844, Centre Hospitalier Régional Universitaire de Tours (CHRU de Tours), Etablissement Français du Sang, U791, and Institut Naltional de la Santé et de la Recherche Médicale
- Subjects
Pathology ,medicine.medical_specialty ,[SDV]Life Sciences [q-bio] ,Cellular differentiation ,Immunology ,Bone Marrow Cells ,Biology ,Biochemistry ,CD49b ,Mice ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,Humans ,CD90 ,Cells, Cultured ,Cell Proliferation ,030304 developmental biology ,Stem cell transplantation for articular cartilage repair ,0303 health sciences ,Cell Membrane ,Mesenchymal stem cell ,Membrane Proteins ,Mesenchymal Stem Cells ,Cell Biology ,Hematology ,Hematopoietic Stem Cells ,Cell biology ,Haematopoiesis ,Phenotype ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Bone marrow ,Stem cell - Abstract
International audience; We have studied the plasma membrane protein phenotype of human culture-amplified and native bone marrow mesenchymal stem cells (BM MSCs). We have found, using microarrays and flow cytometry, that cultured cells express specifically 113 transcripts and 17 proteins that were not detected in hematopoietic cells. These antigens define a lineage-homogenous cell population of mesenchymal cells, clearly distinct from the hematopoietic lineages, and distinguishable from other cultured skeletal mesenchymal cells (periosteal cells and synovial fibroblasts). Among the specific membrane proteins present on cultured MSCs, 9 allowed the isolation from BM mononuclear cells of a minute population of native MSCs. The enrichment in colony-forming units–fibroblasts was low for CD49b, CD90, and CD105, but high for CD73, CD130, CD146, CD200, and integrin alphaV/beta5. In addition, the expression of CD73, CD146, and CD200 was down-regulated in differentiated cells. The new marker CD200, because of its specificity and immunomodulatory properties, deserves further in-depth studies.
- Published
- 2008