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Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells
- Source :
- Blood, Blood, American Society of Hematology, 2008, 111 (5), pp.2631-2635. ⟨10.1182/blood-2007-07-099622⟩
- Publication Year :
- 2008
- Publisher :
- American Society of Hematology, 2008.
-
Abstract
- International audience; We have studied the plasma membrane protein phenotype of human culture-amplified and native bone marrow mesenchymal stem cells (BM MSCs). We have found, using microarrays and flow cytometry, that cultured cells express specifically 113 transcripts and 17 proteins that were not detected in hematopoietic cells. These antigens define a lineage-homogenous cell population of mesenchymal cells, clearly distinct from the hematopoietic lineages, and distinguishable from other cultured skeletal mesenchymal cells (periosteal cells and synovial fibroblasts). Among the specific membrane proteins present on cultured MSCs, 9 allowed the isolation from BM mononuclear cells of a minute population of native MSCs. The enrichment in colony-forming units–fibroblasts was low for CD49b, CD90, and CD105, but high for CD73, CD130, CD146, CD200, and integrin alphaV/beta5. In addition, the expression of CD73, CD146, and CD200 was down-regulated in differentiated cells. The new marker CD200, because of its specificity and immunomodulatory properties, deserves further in-depth studies.
- Subjects :
- Pathology
medicine.medical_specialty
[SDV]Life Sciences [q-bio]
Cellular differentiation
Immunology
Bone Marrow Cells
Biology
Biochemistry
CD49b
Mice
03 medical and health sciences
0302 clinical medicine
medicine
Animals
Humans
CD90
Cells, Cultured
Cell Proliferation
030304 developmental biology
Stem cell transplantation for articular cartilage repair
0303 health sciences
Cell Membrane
Mesenchymal stem cell
Membrane Proteins
Mesenchymal Stem Cells
Cell Biology
Hematology
Hematopoietic Stem Cells
Cell biology
Haematopoiesis
Phenotype
medicine.anatomical_structure
030220 oncology & carcinogenesis
Bone marrow
Stem cell
Subjects
Details
- ISSN :
- 15280020 and 00064971
- Volume :
- 111
- Database :
- OpenAIRE
- Journal :
- Blood
- Accession number :
- edsair.doi.dedup.....d83de78121e2287267aad7b21b340b43