Search

Bone marrow examination of a patient with a myeloproliferative disorder revealed monosomy for chromosome No. 6 (45,XX,-6). Two months later, during blastic crisis, reinvestigation of the bone marrow showed the presence of the Ph-1 chromosome in the previously aneuploid cell line (45,XX,-6,-22,+Ph-1). This case differs from those previously published in that the Ph-1 chromosome appeared de novo during the development of frank acute myelogenous leukemia.

A transferrin-free serum, produced by removing the siderophilin with a specific antitransferrin antibody, was incapable of stimulating the entry of iron into reticulocytes, unless it was reconstituted with pure transferrin-iron complex. These observations militate against the existence of serum factor(s), other than transferrin, capable of stimulating the incorporation of iron into reticulocytes. Comparing the effects of whole serum, pure transferrin, transferrin-free serum, and the latter reconstituted with pure transferrin, it was found that normal, pooled human serum augmented the utilization of intracellular iron for heme production, which transferrin alone did not achieve.