1. Simple chitin-based cell culture platform for production of biopharmaceuticals.
- Author
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Kida K, Hatanaka D, Minami M, Suzuki T, Iwakami M, Kobayashi M, Hayashi H, Kawahara H, Horikawa M, and Kanaki T
- Subjects
- Animals, Cricetinae, Humans, HEK293 Cells, Chitin, CHO Cells, Cricetulus, Cell Culture Techniques, Biological Products
- Abstract
Objectives: Gene therapy using viral vectors and antibody-based therapies continue to expand within the pharmaceutical market. We evaluated whether Cellhesion
® VP, a chitin-based material, can be used as 3D culture platform for cell lines used for the production of antibodies and viral vectors., Results: The results of Cell Counting Kit-8 assay and LDH assay revealed that Cellhesion® VP had no adverse effect to Human Embryonic Kidney (HEK) 293, A549 and Chinese hamster ovary (CHO) DG44-Interferon-β (IFN) cells. Cell growth analyses showed that Cellhesion® VP supported the 3D culture of HEK293, A549 and CHO DG44- IFN-β cells with a spherical morphology. Importantly, subculture of these cell lines on Cellhesion® VP was easily performed without trypsinization because cells readily transferred to newly added scaffold. Our data also suggest that CHO DG44-IFNβ, cultured on Cellhesion® VP secreted IFNβ stably and continuously during the culture period., Conclusions: Cellhesion® VP provides a simple and streamlined expansion culture system for the production of biopharmaceuticals., (© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)- Published
- 2023
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