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Simple chitin-based cell culture platform for production of biopharmaceuticals.

Authors :: Kida K
Hatanaka D
Minami M
Suzuki T
Iwakami M
Kobayashi M
Hayashi H
Kawahara H
Horikawa M
Kanaki T
Source :: Biotechnology letters [Biotechnol Lett] 2023 Oct; Vol. 45 (10), pp. 1265-1277. Date of Electronic Publication: 2023 Aug 22.
Publication Year :: 2023
Abstract: Objectives: Gene therapy using viral vectors and antibody-based therapies continue to expand within the pharmaceutical market. We evaluated whether Cellhesion <superscript>®</superscript> VP, a chitin-based material, can be used as 3D culture platform for cell lines used for the production of antibodies and viral vectors.<br />Results: The results of Cell Counting Kit-8 assay and LDH assay revealed that Cellhesion <superscript>®</superscript> VP had no adverse effect to Human Embryonic Kidney (HEK) 293, A549 and Chinese hamster ovary (CHO) DG44-Interferon-β (IFN) cells. Cell growth analyses showed that Cellhesion <superscript>®</superscript> VP supported the 3D culture of HEK293, A549 and CHO DG44- IFN-β cells with a spherical morphology. Importantly, subculture of these cell lines on Cellhesion <superscript>®</superscript> VP was easily performed without trypsinization because cells readily transferred to newly added scaffold. Our data also suggest that CHO DG44-IFNβ, cultured on Cellhesion <superscript>®</superscript> VP secreted IFNβ stably and continuously during the culture period.<br />Conclusions: Cellhesion <superscript>®</superscript> VP provides a simple and streamlined expansion culture system for the production of biopharmaceuticals.<br /> (© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)