1. Scale‐up of an intensified bioprocess for the expansion of bovine adipose‐derived stem cells (bASCs) in stirred tank bioreactors
- Author
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Mariana P. Hanga, Alvin W. Nienow, Christopher J. Hewitt, Panagiota Moutsatsou, Ivan Wall, and Fritz Anthony de la Raga
- Subjects
0106 biological sciences ,0301 basic medicine ,Cell Culture Techniques ,Adipose tissue ,Bioengineering ,01 natural sciences ,Applied Microbiology and Biotechnology ,03 medical and health sciences ,Laboratory flask ,Bioreactors ,010608 biotechnology ,Bioreactor ,Animals ,Food science ,Bioprocess ,Cell Proliferation ,Chemistry ,Stem Cells ,Microcarrier ,Time optimal ,030104 developmental biology ,Adipose Tissue ,SCALE-UP ,Cattle ,Stem cell ,Biotechnology - Abstract
Cultivated meat is an emerging field, aiming to establish the production of animal tissue for human consumption in an in vitro environment, eliminating the need to raise and slaughter animals for their meat. To realise this, the expansion of primary cells in a bioreactor is needed to achieve the high cell numbers required. The aim of this study was to develop a scalable, microcarrier based, intensified bioprocess for the expansion of bovine adipose-derived stem cells as precursors of fat and muscle tissue. The intensified bioprocess development was carried out initially in spinner flasks of different sizes and then translated to fully controlled litre scale benchtop bioreactors. Bioprocess intensification was achieved by utilising the previously demonstrated bead-to-bead transfer phenomenon and through the combined addition of microcarrier and medium to double the existing surface area and working volume in the bioreactor. Choosing the optimal time point for the additions was critical in enhancing the cell expansion. A significant fold increase of 114.19 ± 1.07 was obtained at the litre scale in the intensified bioprocess compared to the baseline (**p < .005). The quality of the cells was evaluated pre- and post-expansion and the cells were found to maintain their phenotype and differentiation capacity.
- Published
- 2021