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Scale‐up of an intensified bioprocess for the expansion of bovine adipose‐derived stem cells (bASCs) in stirred tank bioreactors
- Source :
- Biotechnology and Bioengineering. 118:3175-3186
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Cultivated meat is an emerging field, aiming to establish the production of animal tissue for human consumption in an in vitro environment, eliminating the need to raise and slaughter animals for their meat. To realise this, the expansion of primary cells in a bioreactor is needed to achieve the high cell numbers required. The aim of this study was to develop a scalable, microcarrier based, intensified bioprocess for the expansion of bovine adipose-derived stem cells as precursors of fat and muscle tissue. The intensified bioprocess development was carried out initially in spinner flasks of different sizes and then translated to fully controlled litre scale benchtop bioreactors. Bioprocess intensification was achieved by utilising the previously demonstrated bead-to-bead transfer phenomenon and through the combined addition of microcarrier and medium to double the existing surface area and working volume in the bioreactor. Choosing the optimal time point for the additions was critical in enhancing the cell expansion. A significant fold increase of 114.19 ± 1.07 was obtained at the litre scale in the intensified bioprocess compared to the baseline (**p < .005). The quality of the cells was evaluated pre- and post-expansion and the cells were found to maintain their phenotype and differentiation capacity.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Cell Culture Techniques
Adipose tissue
Bioengineering
01 natural sciences
Applied Microbiology and Biotechnology
03 medical and health sciences
Laboratory flask
Bioreactors
010608 biotechnology
Bioreactor
Animals
Food science
Bioprocess
Cell Proliferation
Chemistry
Stem Cells
Microcarrier
Time optimal
030104 developmental biology
Adipose Tissue
SCALE-UP
Cattle
Stem cell
Biotechnology
Subjects
Details
- ISSN :
- 10970290 and 00063592
- Volume :
- 118
- Database :
- OpenAIRE
- Journal :
- Biotechnology and Bioengineering
- Accession number :
- edsair.doi.dedup.....1fbed4e10a319c1acb010ad63963d0c7