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The effect of culture media, explants and genotypes on adventitious shoot regeneration in spring rapeseed (Brassica napus L.) was examined. Hypocotyls and cotyledons of the doubled haploid lines NL-611, NL-662, NL-685 were induced to form callus by culturing on the Murashige- Skoog medium supplemented with different concentrations of 6-benzylaminopurine (BAP) and α-naphtylacetic acid (NAA) or zeatin and 2,4-dichlorphenoxyacetic acid (2,4-D). Adventitious buds were regenerated from the organogenic callus on the same medium. A large variation of shoot regenerability was observed, ranging within 0-37.5% for the frequency of bud formation and within 0-3.8 for the number of buds per explant. Generally, cotyledon-derived callus exhibited a higher bud regeneration frequency than hypocotyls, however, hypocotyl-derived callus developed a higher number of buds per explant. The maximum number of adventitious buds from hypocotyl-derived callus was obtained on a medium supplemented with 4.0 mg l-1 BAP and 0.05 mg l-1 NAA. Regenerated shoots were rooted in MS medium containing 0.1 mg l-1 NAA. Well rooted plantlets were acclimatized and subsequently established in soil. [ABSTRACT FROM AUTHOR]

Genus Potentilla L. is presented in Crimea by 20 species, including two endemic P. taurica Willd. ex Schlecht. and P. depressa Willd. Many of Potentilla species are so ornamental and due to the secondary metabolites are used in various industries. To study the accumulation of biologically active substances and conservation of biodiversity the morphogenetic capacity of organs and tissues in vitro of some Potentilla species has been identified. As explants the leaves, petioles, meristems, roots of P. depressa, P. recta subsp. laciniosa (Waldst. et Kit. ex Nestler) Nyman, P. inclinata Vill., internodes and seeds of P. recta have been taken. For plant material sterilization 70-96% ethanol and 0.375-1.125% Cl2 (Dez Tab, Ukraine) have been used. The sterilization of P. recta seeds 1 min by 70% ethanol and 10 min by 0.375% Cl2 provided possibility to obtain 71.4% aseptic explants, from which 40% have been viable. Plants from seeds on Monnier medium have been developed. Thus on 1/2 Murashige & S koog medium with 0.4-0.8 mg l-1 BAP and 0.05-0.1 mg l-1 IBA microshoots with small leaves have been formed. From root explants, leaf pieces, petioles of P. depressa, P. recta and P. inclinata callus have been formed on MS medium, supplemented with 0.2-0.6 mg l-1 kinetin and 0.8-1.2 mg l-1 NAA, 0.4-0.8 mg l-1 BA and 0.05-0.1 mg l-1 IBA, 1-1.5 mg l-1 BA and 1-1.5 mg l-1 NAA. On the internodes on MS medium with 0.2-0.6 mg l-1 kinetin and 0.8-1.2 mg l-1 NAA indirect regeneration has been induced. [ABSTRACT FROM AUTHOR]

Introduction and propagation of valuable species and cultivars, conservation of plant biodiversity are the main tasks of botanical gardens, both in Ukraine and abroad. The main biotechnology methods of plant conservation are cryopreservation and storage of plants in the form of slow-growing collection of in vitro (germplasm preservation). For creation of plant growing collection in vitro 32 rose cultivars (Rosa sp.) of 9 garden groups, 2 species and 2 cultivars of orchid (Cymbidium sp. and Dossinia sp.), 16 cultivars of clematis (Clematis sp.), 2 species of yucca (Yucca aloifolia, Y. torreyi), 2 forms of feijoa (Feijoa sellowiana), 4 cultivars of kiwifruit (Actinidia deliciosa), 9 cultivars of sweet plum (Prunus cerasus), plum (Prunus domestica), apricot (Prunus armeniaca) and peach (Prunus persica) have been selected from the collection of Nikitsky Botanical Garden--National Scientific Centre (NBG-NSC, Yalta, Ukraine). At the beginning for each species and cultivars the methods of somatic embryogenesis and organogenesis have been developed. As explants the microshoots of roses, clematis, orchids, yuccas, peach, apricot, plum, prune, kiwi, feijoa, protocorms of orchids and somatic embryos of clematis have been used. High viability of explants during 36 months of conservation with active regeneration capacity after transferring to standard conditions have been obtained due to optimal factors, such as temperature 5 ± 1 °C, light intensity 1.25 μM m-2 s-1, ½ Murashige & S koog, Gamborg & E veleigh, Quoirin & Lepoivre and Knudson C mediums, supplemented with 60-90 g l-1 sucrose and 0.2-0.4 ml l-1 CCC . [ABSTRACT FROM AUTHOR]