INVESTIGATION OF THE GENUS POTENTILLA L. IN CRIMEA AND DEVELOPMENT OF IN VITRO PROPAGATION METHODS.

Genus Potentilla L. is presented in Crimea by 20 species, including two endemic P. taurica Willd. ex Schlecht. and P. depressa Willd. Many of Potentilla species are so ornamental and due to the secondary metabolites are used in various industries. To study the accumulation of biologically active substances and conservation of biodiversity the morphogenetic capacity of organs and tissues in vitro of some Potentilla species has been identified. As explants the leaves, petioles, meristems, roots of P. depressa, P. recta subsp. laciniosa (Waldst. et Kit. ex Nestler) Nyman, P. inclinata Vill., internodes and seeds of P. recta have been taken. For plant material sterilization 70-96% ethanol and 0.375-1.125% Cl2 (Dez Tab, Ukraine) have been used. The sterilization of P. recta seeds 1 min by 70% ethanol and 10 min by 0.375% Cl2 provided possibility to obtain 71.4% aseptic explants, from which 40% have been viable. Plants from seeds on Monnier medium have been developed. Thus on 1/2 Murashige & S koog medium with 0.4-0.8 mg l-1 BAP and 0.05-0.1 mg l-1 IBA microshoots with small leaves have been formed. From root explants, leaf pieces, petioles of P. depressa, P. recta and P. inclinata callus have been formed on MS medium, supplemented with 0.2-0.6 mg l-1 kinetin and 0.8-1.2 mg l-1 NAA, 0.4-0.8 mg l-1 BA and 0.05-0.1 mg l-1 IBA, 1-1.5 mg l-1 BA and 1-1.5 mg l-1 NAA. On the internodes on MS medium with 0.2-0.6 mg l-1 kinetin and 0.8-1.2 mg l-1 NAA indirect regeneration has been induced. [ABSTRACT FROM AUTHOR]