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Deals with a study which examined the tertiary contacts between helices V and VII, VIII or X using a general cross-linking approach in the lactose permease. Experimental procedures; Results of the study; Discussion of the results.

Highlights a study which explored the N-terminal six transmembrane helices and the C-terminal transmembrane helices of the lactose permease, containing a single-Cys residue. Methodology; Cross-linking permease with paired Cys residues in helices IV and VII; Ligand-induced changes in cross-linking.