1. Molecular Cloning and Expression Analysis of Porcine Ghrelin O-Acyltransferase
- Author
-
Qingyong Meng, Ning Li, Yang Li, Dezhi Peng, Xiaofang Liu, Longfei Xie, Tonghui Lin, and Dandan Sui
- Subjects
Male ,Untranslated region ,medicine.medical_specialty ,Molecular Sequence Data ,Sus scrofa ,Gene Expression ,Peptide hormone ,Biology ,Biochemistry ,Catalytic Domain ,Internal medicine ,Complementary DNA ,Gene expression ,Genetics ,medicine ,Animals ,Amino Acid Sequence ,Cloning, Molecular ,Molecular Biology ,Phylogeny ,Ecology, Evolution, Behavior and Systematics ,Messenger RNA ,Base Sequence ,Sequence Homology, Amino Acid ,digestive, oral, and skin physiology ,Sequence Analysis, DNA ,General Medicine ,Recombinant Proteins ,Ghrelin O-acyltransferase ,Open reading frame ,Endocrinology ,Organ Specificity ,Female ,Ghrelin ,Acyltransferases - Abstract
The peptide hormone ghrelin is secreted in the stomach, with unique N-octanoylation at serine 3, which is a requirement for its functionality. These functions include growth hormone release, appetite stimulation, gastrointestinal motility, glucose regulation, and cell proliferation. The enzyme responsible for ghrelin acylation was recently identified as ghrelin O-acyltransferase (GOAT). In this study, porcine GOAT was cloned and characterized. A full-length cDNA of GOAT of 2013 bp was obtained, which included a 70-bp 5' UTR, a 635-bp 3' UTR, and a 1308-bp open reading frame encoding a protein of 415 amino acids. The GOAT and ghrelin mRNAs are co-expressed in stomach, pancreas, and duodenum at high levels. GOAT was also detected in liver, lung, brain, testis, spleen, kidney, heart, muscle, lipid, and ovary. Our results provide an important basis for further research on GOAT function and the relationship between ghrelin and GOAT.
- Published
- 2011