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This study has been conducted at College of Agriculture, university of Baghdad with cooperation of High Institute of Infertility Diagnosis and Assisted Reproductive Technology, Al-Nahrain University and Ministry of Agriculture, Agricultural Research Center to investigate the effect of adding different concentration of selenium and cysteine to the ram semen extender to enhance the post freezing semen characteristics. Four awassi ram were used in this study, the semen was collected with artificial vagina as one ejaculate/ram/week, The microscope tests of semen were processed before cooling, and the tris solution was used to dilute samples as the percentage of 1:1 as well. The Semen samples were pooling and the final dilution as the percentage of 1:10 was completed. the samples were then divided into seven parts, control, cysteine (5,10,15mmol) and selenium( 1,2,4μg) in 5C and the dilution completed to 1:20 with adding glycerol 5% to each 100ml of dilution. all these would be left 2 hours to get the equilibrium periods, then sample exposed to the nitrogen vapor for 10 minutes before immersed in liquid nitrogen. After two months the sample would levered up and thawed in water bath. The results could be briefed as follows: cysteine 5mmol and selenium 1μg overlapped all others treatments in all studying characteristics (sperm motility, plasma integrity, dead sperm, DNA fragmentation and mitochondrial apoptosis) compared to other concentrations. [ABSTRACT FROM AUTHOR]

The main objective of this study is to investigate the effect of both time (1-2-3) and temperature (15-30- 37°C) during the transfer of ovaries from the slaughterhouse to the laboratory on the percentages of mortality and abnormality of oocytes and ovarian follicles. This study was performed using ewes slaughtered in the local slaughterhouse. The study was conducted at the College of Agriculture, University of Qadisiyah. 130 sheep ovaries were collected and the mortality percentages of oocytes and follicles were assessed using the trypan blue stain. The percentage of abnormality was also calculated in oocytes. Histological sections were carry out to recorded abnormality percentages in ovarian follicles. The results of this study showed a significant decrease (P d"0.05) in the percentage of oocytes and ovarian follicles abnormalities in the transport of ovaries in one hour and under 37 ° C, while the duration of transport 3 hours and 15°C showed the highest percentage of abnormality and mortality of oocytes. primordial, primary and secondary ovarian follicles mortality and abnormality percentages recorded lest percentage when transported ovaries in one hour and under 15°C, while recorded transport time 3 hours and 15 ° C higher percentage of primary ovarian follicles and (P £0.05) in the percentage of abnormality of tertiary ovarian follicles, while the transport time at 1 h and 37°C showed significantly decrease(P £0.05) in the percentage of the tertiary ovarian follicles. In this study, we conclude that the temperature of 37°C and one hour was the best in the transfer of ovaries to the vitality and deformities of oocytes and ovarian follicles while the temperature of 150C and one hour for the best rate of vitality and abnormality of ovarian follicular pre-vesicular fluid. [ABSTRACT FROM AUTHOR]