IMPACT OF BOTH OF TIME AND TEMPERATURE OF OVINE OVARIES TRANSPORT IN MORTALITY AND ABNORMALITY PERCENTAGES OF OOCYTES AND OVARIAN FOLLICLES.

The main objective of this study is to investigate the effect of both time (1-2-3) and temperature (15-30- 37°C) during the transfer of ovaries from the slaughterhouse to the laboratory on the percentages of mortality and abnormality of oocytes and ovarian follicles. This study was performed using ewes slaughtered in the local slaughterhouse. The study was conducted at the College of Agriculture, University of Qadisiyah. 130 sheep ovaries were collected and the mortality percentages of oocytes and follicles were assessed using the trypan blue stain. The percentage of abnormality was also calculated in oocytes. Histological sections were carry out to recorded abnormality percentages in ovarian follicles. The results of this study showed a significant decrease (P d"0.05) in the percentage of oocytes and ovarian follicles abnormalities in the transport of ovaries in one hour and under 37 ° C, while the duration of transport 3 hours and 15°C showed the highest percentage of abnormality and mortality of oocytes. primordial, primary and secondary ovarian follicles mortality and abnormality percentages recorded lest percentage when transported ovaries in one hour and under 15°C, while recorded transport time 3 hours and 15 ° C higher percentage of primary ovarian follicles and (P £0.05) in the percentage of abnormality of tertiary ovarian follicles, while the transport time at 1 h and 37°C showed significantly decrease(P £0.05) in the percentage of the tertiary ovarian follicles. In this study, we conclude that the temperature of 37°C and one hour was the best in the transfer of ovaries to the vitality and deformities of oocytes and ovarian follicles while the temperature of 150C and one hour for the best rate of vitality and abnormality of ovarian follicular pre-vesicular fluid. [ABSTRACT FROM AUTHOR]