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1. Paper-based microfluidic device for diagnosis of osteoporosis markers.

Author

R Yerrapragada M and Narayanan Unni H

Subjects
Calibration, Colorimetry instrumentation, Equipment Design, Humans, Osteoporosis diagnosis, Point-of-Care Systems, Sensitivity and Specificity, Smartphone, Alkaline Phosphatase blood, Calcium blood, Lab-On-A-Chip Devices, Osteoporosis blood, Paper, Vitamin D blood
Abstract

Background: Development of cost-effective platforms for identification of biomarkers is of paramount importance in low-income settings. The present work focuses on the development of a microfluidic paper-based analytical device (μPADs) for the diagnosis of osteoporosis by measuring three important bone biomarkers - calcium, alkaline phosphatase and vitamin D., Methods: Antibody-based detection is realized in μPAD reservoirs based on principle of colorimetric sensing, where μPAD images are captured using a smartphone. The results are compared with the calibration curves to give proper quantification of analytes., Conclusion: This device can be potentially developed into a point-of-care diagnostic device for low-income settings. To the best of our understanding, a μPAD platform for simultaneous diagnosis of multiple osteoporosis markers is not reported yet.

Published
2018
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2. Paper-based microfluidic device for diagnosis of osteoporosis markers

Author

Manjoosha R Yerrapragada and Harikrishnan Narayanan Unni

Subjects
Paper, Computer science, Point-of-Care Systems, Clinical Biochemistry, Microfluidics, Biomedical Engineering, 02 engineering and technology, 01 natural sciences, Sensitivity and Specificity, Analytical Chemistry, Lab-On-A-Chip Devices, Humans, General Pharmacology, Toxicology and Pharmaceutics, Vitamin D, Point of care, 010401 analytical chemistry, General Medicine, Paper based, Equipment Design, 021001 nanoscience & nanotechnology, Alkaline Phosphatase, 0104 chemical sciences, Medical Laboratory Technology, Calibration, Systems engineering, Osteoporosis, Identification (biology), Calcium, Colorimetry, Smartphone, 0210 nano-technology
Abstract

Background: Development of cost-effective platforms for identification of biomarkers is of paramount importance in low-income settings. The present work focuses on the development of a microfluidic paper-based analytical device (μPADs) for the diagnosis of osteoporosis by measuring three important bone biomarkers – calcium, alkaline phosphatase and vitamin D. Methods: Antibody-based detection is realized in μPAD reservoirs based on principle of colorimetric sensing, where μPAD images are captured using a smartphone. The results are compared with the calibration curves to give proper quantification of analytes. Conclusion: This device can be potentially developed into a point-of-care diagnostic device for low-income settings. To the best of our understanding, a μPAD platform for simultaneous diagnosis of multiple osteoporosis markers is not reported yet.

Published
2018