1. Association analysis of polymorphisms in six keratin genes with wool traits in sheep
- Author
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Mahira Tursun, Tian Yuezhen, Fu Xuefeng, Yiming Sulaiman, Kechuan Tian, Ablat Sulayman, Xu Xinming, Hanikezi Tulafu, Amat Mamat, and Huang Xixia
- Subjects
0301 basic medicine ,Silent mutation ,Candidate gene ,Wool Traits ,Population ,Nonsense mutation ,lcsh:Animal biochemistry ,Locus (genetics) ,Biology ,Article ,03 medical and health sciences ,Genotype ,Polymorphism ,education ,lcsh:QP501-801 ,Gene ,lcsh:SF1-1100 ,Genetic association ,Genetics ,Chinese Merino (Xinjiang Type) ,education.field_of_study ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,Animal Breeding and Genetics ,040201 dairy & animal science ,030104 developmental biology ,Keratin ,Combined Genotype ,Animal Science and Zoology ,lcsh:Animal culture ,Food Science - Abstract
Objective The purpose of this study was to investigate the genetic effects of six keratin (KRT) genes on the wool traits of 418 Chinese Merino (Xinjiang type) (CMXT) individuals. Methods To explore the effects and association of six KRT genes on sheep wool traits, The polymerase chain reaction-based single-strand conformation polymorphism (PCR-SSCP), DNA sequencing, and the gene pyramiding effect methods were used. Results We report 20 mutation sites (single-nucleotide polymorphisms) within the six KRT genes, in which twelve induced silent mutations; five induced missense mutations and resulted in Ile→Thr, Glu→Asp, Gly→Ala, Ala→Ser, Se→His; two were nonsense mutations and one was a same-sense mutation. Association analysis showed that two genotypes of the KRT31 gene were significantly associated with fiber diameter (p
- Published
- 2018