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8 results on '"Venkitasubramanian TA"'

1. Study of glycogen synthesizing and degrading enzymes of guinea pig liver in experimental tuberculosis

Author

Vishwa Nath Singh, Venkitasubramanian Ta, R. Viswanathan, and Usha Bhargava

Subjects
medicine.medical_specialty, Guinea Pigs, Phosphatase, Biophysics, Biology, Biochemistry, Guinea pig, Glycogen phosphorylase, chemistry.chemical_compound, Glucosyltransferases, Internal medicine, medicine, Tuberculosis, Molecular Biology, chemistry.chemical_classification, Glycogen, Phosphotransferases, Phosphoric Monoester Hydrolases, Liver Glycogen, Experimental tuberculosis, Endocrinology, Enzyme, Liver, Phosphoglucomutase, chemistry
Abstract

Enzymes of the glycogen cycle, namely, UDPG-glycogen transglucosylase, phosphorylase, phosphoglucomutase, and glucose-6-phosphatase, were assayed in the liver of normal and tuberculous guinea pigs. A significant decrease in the activities of the first three enzymes and increase in that of the last one were noted. The decrease in the activity of UDPG-glycogen transglucosylase was of significant magnitude to account for the depletion of the hepatic glycogen in tuberculous guinea pigs.

Published
1963
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2. Purification and properties of pyruvate kinase from Mycobacterium smegmatis.

Author

Kapoor R and Venkitasubramanian TA

Subjects
Adenosine Triphosphate pharmacology, Amino Acids analysis, Cations pharmacology, Chemical Phenomena, Chemistry, Hot Temperature, Hydrogen-Ion Concentration, Kinetics, Molecular Weight, Phosphoenolpyruvate metabolism, Pyruvate Kinase antagonists & inhibitors, Mycobacterium enzymology, Pyruvate Kinase isolation & purification
Abstract

Pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) from Mycobacterium smegmatis has been purified to homogeneity through a seven-step procedure with a yield of 16% and specific activity of 220 units/mg protein. The purified enzyme had a molecular weight of 230,700 and was composed of four subunits with identical molecular weights of 57,540. Analysis of amino acid composition revealed a low content of aromatic amino acids. The enzyme exhibited sigmoidal kinetics of varying concentrations of phosphoenolpyruvate, the degree of cooperativity and S0.5v value for phosphoenolpyruvate being strongly dependent on the pH of the reaction mixture. Among the nucleoside diphosphates acting as substrate for pyruvate kinase, ADP was the best phosphate acceptor, as judged by its lowest Km value. The enzyme showed an absolute requirement for divalent cations (either Mg2+ or Mn2+), but monovalent cations were not necessary for activity. Other divalent cations inhibited the Mg2+-activated enzyme to varying degrees (Ni2+ greater than Zn2+ greater than Cu2+ greater than Ca2+ greater than Ba2+). The differences in the kinetic responses of the enzyme to Mg2+ and Mn2+ are discussed.

Published
1983
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3. Early effects of excessive retinol intake on gluconeogenesis. Involvement of adrenals in the increased activities on Gluconeogenic Enzymes of rat.

Author

Singh M, Singh VN, and Venkitasubramanian TA

Subjects
Adrenalectomy, Alanine Transaminase metabolism, Animals, Aspartate Aminotransferases metabolism, Bicarbonates metabolism, Blood Glucose metabolism, Dactinomycin pharmacology, Fructose-Bisphosphatase metabolism, Glucose-6-Phosphatase metabolism, Glycine metabolism, Liver drug effects, Liver Glycogen metabolism, Male, Phosphoenolpyruvate Carboxykinase (GTP) metabolism, Rats, Adrenal Glands physiology, Gluconeogenesis drug effects, Liver metabolism, Vitamin A analogs & derivatives, Vitamin A pharmacology
Published
1976
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4. Fructose diphosphate aldolase from Mycobacterium smegmatis. Functional similarities with rabbit muscle aldolase.

Author

Jayanthi Bai N, Ramachandra Pai M, Suryanarayana Murthy P, and Venkitasubramanian TA

Subjects
Animals, Binding Sites, Carboxypeptidases, Chloromercuribenzoates pharmacology, Dithionitrobenzoic Acid pharmacology, Electrophoresis, Disc, Ethylmaleimide pharmacology, Iodoacetamide pharmacology, Kinetics, Light, Macromolecular Substances, Methylene Blue pharmacology, Phenanthrolines pharmacology, Protein Binding, Rabbits, Species Specificity, Fructose-Bisphosphate Aldolase metabolism, Muscles enzymology, Mycobacterium enzymology
Published
1975
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6. Fructose diphosphate aldolase from Mycobacterium smegmatis. Purification and properties.

Author

Jayanthi Bia N, Ramachandra Pai M, Suryanarayana Murthy P, and Venkitasubramanian TA

Subjects
Chromatography, DEAE-Cellulose, Chromatography, Gel, Electrophoresis, Cellulose Acetate, Electrophoresis, Polyacrylamide Gel, Fructose-Bisphosphate Aldolase metabolism, Kinetics, Molecular Weight, Fructose-Bisphosphate Aldolase isolation & purification, Mycobacterium enzymology
Published
1975
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