1. The intestinal microbiota of piglets fed with wheat bran variants as characterised by 16S rRNA next-generation amplicon sequencing
- Author
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Karl Schedle, Manuel Kraler, Konrad J. Domig, Wolfgang Kneifel, and Mahdi Ghanbari
- Subjects
Dietary Fiber ,0301 basic medicine ,Firmicutes ,Sus scrofa ,Ileum ,Context (language use) ,Biology ,Microbiology ,Jejunum ,Random Allocation ,03 medical and health sciences ,RNA, Ribosomal, 16S ,Lactobacillus ,medicine ,Prevotella ,Animals ,Food science ,Bifidobacterium ,General Veterinary ,Bran ,digestive, oral, and skin physiology ,Sequence Analysis, DNA ,General Medicine ,biology.organism_classification ,Animal Feed ,Diet ,Gastrointestinal Microbiome ,030104 developmental biology ,medicine.anatomical_structure ,Animal Nutritional Physiological Phenomena ,Animal Science and Zoology - Abstract
The intestinal microbiota of piglets fed with a Control diet low in dietary fibre and modified wheat bran variants as an additional source of insoluble dietary fibre was characterised. In this context, variances in the microbiota of three different gut segments were assessed. Wheat bran was either included in its native form or modified by fermentation and extrusion before added at 150 g/kg to a basal diet for 48 piglets (12 animals per treatment). Total DNA was extracted from digesta samples from the jejunum, the end of the ileum and the colon ascendens. Samples were prepared accordingly for subsequent sequencing with the Illumina MiSeq. The obtained results revealed distinct location-specific differences in microbial composition. While Firmicutes were most predominant in all three gut segments, Bacteroidetes were additionally found in the colon at high abundance. The parameters of alpha and beta diversity analysis showed significant differences (p
- Published
- 2016